{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T07:31:52Z","timestamp":1664177512787},"reference-count":35,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>Albuminuria in nephrotic states is thought to arise from the formation of large pores in the glomerular capillary wall as large hydrodynamic probes, like Ficoll, have increased fractional clearance. In the present study, we tested for large pore formation in a novel manner. We accounted for the rates of plasma elimination as determined for tritium-labeled tracers of uncharged polydisperse Ficoll (radii range: 35\u201385 \u00c5) and two globular<jats:sup>14<\/jats:sup>C-labeled proteins, albumin (radius: 36 \u00c5) and IgG (radius: 55 \u00c5), in control and puromycin aminonucleoside nephrotic (PAN) Sprague-Dawley rats. The plasma elimination rates were then matched to the urinary excretion of these labeled materials ( n = 7). Albumin and IgG plasma retention rates were identical and far enhanced compared with the retention rates of inert transport markers of equivalent hydrodynamic radius; their elimination rate corresponded to the elimination of a 75-\u00c5 radius Ficoll ( n = 5) and &gt;105-\u00c5 radius dextran ( n = 5). In PAN, they were eliminated as \u223c36- and \u223c55-\u00c5 radii Ficoll, respectively, equivalent to their hydrodynamic radii. In contrast, there was no comparable increase in the elimination rate of Ficoll in PAN. The total plasma clearance of Ficoll in control and PAN rats and the urinary clearance in PAN rats were essentially the same for all radii. On the other hand, the urinary clearance of &gt;45-\u00c5 radii Ficoll in controls was considerably lower with increasing radii, demonstrating a postfiltration cellular uptake in controls, which, when inhibited in nephrotic states, would account for apparent large pore formation.<\/jats:p>","DOI":"10.1152\/ajprenal.00228.2014","type":"journal-article","created":{"date-parts":[[2014,7,3]],"date-time":"2014-07-03T07:25:53Z","timestamp":1404372353000},"page":"F525-F532","source":"Crossref","is-referenced-by-count":6,"title":["Do large pores in the glomerular capillary wall account for albuminuria in nephrotic states?"],"prefix":"10.1152","volume":"307","author":[{"given":"Julijana","family":"Vuchkova","sequence":"first","affiliation":[{"name":"Biochemistry Department, Monash University, Clayton, Victoria, Australia; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Koltun","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Monash University, Clayton, Victoria, Australia; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerryn","family":"Greive","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Monash University, Clayton, Victoria, Australia; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne D.","family":"Comper","sequence":"additional","affiliation":[{"name":"SalAqua Diagnostics, Incorporated, New York, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00183.2011"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1997.273.3.F430"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.74.5.583"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(75)85863-2"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1340"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2013.867334"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00142.2008"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e32832cb96a"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013090950"},{"key":"B10","first-page":"503","volume-title":"Clinical Chemistry Principles and Techniques","author":"Di Giorgio J","year":"1974"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00159.2002"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(85)90209-1"},{"key":"B13","first-page":"59","volume":"28","author":"Granath KA","year":"1966","journal-title":"J Chromatogr"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61792-0"},{"key":"B16","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/S0021-9258(18)57021-6","volume":"177","author":"Gornall AG","year":"1949","journal-title":"J Biol Chem"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00055.2006"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(86)90120-5"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.45.1.201"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1984.247.1.F192"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1982.243.4.F372"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.060003957.x"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00808.2004"},{"key":"B24","first-page":"3005","volume-title":"The Kidney: Physiology and Pathophysiology","author":"Maack T","year":"1992","edition":"3"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(96)00349-8"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/14.8.1898"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.277.1.F139"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00271.2007"},{"key":"B29","unstructured":"Rowland M, Tozer TN.Clinical Pharmacokinetics: Concepts and Applications. 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