{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:18:15Z","timestamp":1761059895018},"reference-count":44,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:p> Ion channels that are gated in response to membrane deformation or \u201cstretch\u201d are empirically designated stretch-activated channels. Here we describe a stretch-activated nonselective cation channel in the basolateral membrane (BLM) of the proximal tubule (PT) that is nucleotide sensitive. Single channels were studied in cell-intact and cell-free patches from the BLM of PT cells that maintain their epithelial polarity. The limiting inward Cs<jats:sup>+<\/jats:sup> conductance is \u223c28 pS, and channel activity persists after excision into a Ca<jats:sup>2+<\/jats:sup>- and ATP-free bath. The stretch-dose response is sigmoidal, with half-maximal activation of about \u221219 mmHg at \u221240 mV, and the channel is activated by depolarization. The inward conductance sequence is: NH[Formula: see text] \u223c Cs<jats:sup>+<\/jats:sup> \u223c Rb<jats:sup>+<\/jats:sup>&gt; K<jats:sup>+<\/jats:sup> \u223c Na<jats:sup>+<\/jats:sup> \u223c Li<jats:sup>+<\/jats:sup> &gt; Ca<jats:sup>2+<\/jats:sup> \u223c Ba<jats:sup>2+<\/jats:sup>&gt; N-methyl-d-glucamine \u223c tetraethylammonium. The venom of the common Chilean tarantula, Grammostola spatulata, completely blocks channel activity in cell-attached patches. Hypotonic swelling reversibly activates the channel. Intracellular ATP concentration ([ATP]<jats:sub>i<\/jats:sub>) reversibly blocks the channel (inhibitory constant \u223c0.48 mM), suggesting that channel function is coupled to the metabolic state of the cell. We conclude that this channel may function as a Ca<jats:sup>2+<\/jats:sup> entry pathway and\/or be involved in regulation of cell volume. We speculate this channel may be important when [ATP]<jats:sub>i<\/jats:sub> is depleted, as occurs during periods of increased transepithelial transport or with ischemic injury. <\/jats:p>","DOI":"10.1152\/ajprenal.00239.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:32:35Z","timestamp":1425414755000},"page":"F93-F104","source":"Crossref","is-referenced-by-count":15,"title":["A mechanogated nonselective cation channel in proximal  tubule that is ATP sensitive"],"prefix":"10.1152","volume":"283","author":[{"given":"Craig G.","family":"Hurwitz","sequence":"first","affiliation":[{"name":"Departments of Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian Y.","family":"Hu","sequence":"additional","affiliation":[{"name":"Departments of Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan S.","family":"Segal","sequence":"additional","affiliation":[{"name":"Departments of Medicine 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