{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T09:14:53Z","timestamp":1776417293861,"version":"3.51.2"},"reference-count":17,"publisher":"American Physiological Society","issue":"5","funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH-17-1-0403"],"award-info":[{"award-number":["W81XWH-17-1-0403"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2019,11,1]]},"abstract":"<jats:p>\n                    We investigated the involvement of brain-derived neurotrophic factor (BDNF) in bladder and urethral dysfunction using spinal cord-injured mice. We evaluated bladder and urethral function of female mice with 4-wk spinal cord injury (SCI) by filling cystometry and electromyography (EMG) of the external urethral sphincter (EUS) under a conscious condition. Anti-BDNF antibodies (10 \u03bcg\u00b7kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) were administered in some mice for 1 wk before the evaluation. Bladder and spinal (L6\u2212S1) BDNF protein levels were examined by ELISA. Transcript levels of transient receptor potential channels or acid-sensing ion channels (Asic) in L6\u2212S1 dorsal root ganglia were evaluated by RT-PCR. Voided volume and voiding efficiency were significantly increased without any changes in nonvoiding contractions, and the duration of reduced EMG activity during the voiding phase was significantly prolonged in anti-BDNF antibody-treated SCI mice. Compared with spinal cord-intact mice, SCI mice showed increased concentrations of bladder and spinal BDNF. Anti-BDNF antibody treatment decreased bladder and spinal BDNF protein concentrations of SCI mice. Asic2 and Asic3 transcripts were significantly increased after SCI but decreased after anti-BDNF antibody administration. These results indicate that upregulated expression of bladder and spinal BDNF is involved in the emergence of inefficient voiding in SCI mice. Thus, BDNF-targeting treatment could be an effective modality for the treatment of voiding problems, including inefficient voiding and detrusor sphincter dyssynergia after SCI.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00239.2019","type":"journal-article","created":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T10:24:06Z","timestamp":1569839046000},"page":"F1305-F1310","source":"Crossref","is-referenced-by-count":22,"title":["Therapeutic effects of inhibition of brain-derived neurotrophic factor on voiding dysfunction in mice with spinal cord injury"],"prefix":"10.1152","volume":"317","author":[{"given":"Naoki","family":"Wada","sequence":"first","affiliation":[{"name":"Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"},{"name":"Department of Renal and Urologic Surgery, Asahikawa Medical University, Asahikawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobutaka","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Kurobe","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"de Groat","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradeep","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiro","family":"Kakizaki","sequence":"additional","affiliation":[{"name":"Department of Renal and Urologic Surgery, Asahikawa Medical University, Asahikawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8070-1664","authenticated-orcid":false,"given":"Naoki","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania"},{"name":"Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, 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