{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:42:11Z","timestamp":1792676531583,"version":"4.5.0"},"reference-count":22,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:p>\n                    Posttraumatic microalbuminuria may be caused by either charge- or size-selective alterations in the glomerular filtration barrier, or both, and\/or to a reduction in proximal tubular protein reabsorption. This study was performed to elucidate the pathophysiology of the increases in glomerular permeability occurring in rats exposed to a laparotomy or to a laparotomy and muscle trauma. In anesthetized Wistar rats (250\u2013280 g), the left ureter was cannulated for urine collection, while simultaneously blood access was achieved. Rats were exposed to trauma by a laparotomy (L; n = 8), or by a combination of L and muscle trauma (MT; L+MT) induced by topical blunt injury of the abdominal muscles bilaterally. After L, muscles were crushed using hemostatic forceps at either 2 \u00d7 2 sites (\u201csmall\u201d MT; n = 9), or at 2 \u00d7 5 sites (\u201clarge\u201d MT; n = 9). Sham groups ( n = 16), not exposed to a laparotomy, were used as controls. The glomerular sieving coefficients (\u03b8) to polydisperse FITC-Ficoll-70\/400 (molecular radius 13\u201380 \u00c5) were determined at 5 or 60 min after L and L+MT, respectively, from plasma and urine samples, and analyzed by high-performance size-exclusion chromatography. A tissue-uptake technique was used to assess \u03b8 for\n                    <jats:sup>125<\/jats:sup>\n                    I-labeled serum albumin. L, with or without MT, increased \u03b8 for Ficoll\n                    <jats:sub>55\u201380\u00c5<\/jats:sub>\n                    and albumin rapidly and markedly. \u03b8-Ficoll\n                    <jats:sub>70\u00c5<\/jats:sub>\n                    thus increased approximately threefold, and \u03b8 for albumin significantly, for all trauma groups. According to the \u201ctwo-pore model\u201d of glomerular permeability, these changes mainly reflect an increase in the number of large pores in the glomerular filter without any primary changes in the charge-selective properties of the filter.\n                  <\/jats:p>","DOI":"10.1152\/ajprenal.00246.2009","type":"journal-article","created":{"date-parts":[[2009,7,8]],"date-time":"2009-07-08T21:51:52Z","timestamp":1247089912000},"page":"F577-F582","source":"Crossref","is-referenced-by-count":27,"title":["Loss of size selectivity of the glomerular filtration barrier in rats following laparotomy and muscle trauma"],"prefix":"10.1152","volume":"297","author":[{"given":"Josefin","family":"Axelsson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irma","family":"Mahmutovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Rippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengt","family":"Rippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00488.2005"},{"key":"R2","unstructured":"Asgerisson D\n                      , Rippe C, Rippe A, Rippe B.\n                      Glomerular sieving of Ficoll compared with that of proteins in paraaminonucleoside (PAN) induced nephrosis in rats (Abstract).\n                      J Am Soc Nephrol\n                      17\n                      : 717A, 2006."},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Axelsson J\n                      , Rippe A, Venturoli D, Sw\u00e4rd P, Rippe B.\n                      Effects of early endotoxemia and dextran-induced anaphylaxis on the size selectivity of the glomerular filtration barrier in rats.\n                      Am J Physiol Renal Physiol\n                      296\n                      : F242\u2013F248, 2009.","DOI":"10.1152\/ajprenal.90263.2008"},{"key":"R4","unstructured":"Bansch P\n                      , Lundblad C, Gr\u00e4nde PO, Bentzer P.\n                      A trauma model for evaluation of the effects of blunt skeletal muscle trauma on microvascular permeability, plasma volume and cytokine release in the rat.\n                      Shock.\n                      In press."},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800840707"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.296.6618.338"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199404000-00002"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00055.2006"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1042\/cs0880629"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00316.2002"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.11.3.571"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1080\/713773924"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.3109\/17453679709003972"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00016.x"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(00)00149-2"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1994.74.1.163"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000027"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00123.2006"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00271.2007"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00797.x"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00171.2004"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2006.02.019"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00246.2009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:09:39Z","timestamp":1567973379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00246.2009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1152\/ajprenal.00246.2009"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00246.2009","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9]]}}}