{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:30:24Z","timestamp":1790861424912,"version":"4.1.0"},"reference-count":54,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p>Apoptosis has been shown to contribute to the development of acute and chronic renal failure. The antiapoptotic action of the heme oxygenase (HO) system may represent an important protective mechanism in kidney pathology. We examined whether the lack of HO-1 would influence apoptosis in clipped kidneys of two-kidney, one-clip (2K1C) rats. Five-day-old Sprague-Dawley rats were injected in the left ventricle with \u22485 \u00d7 10<jats:sup>9<\/jats:sup>colony-forming units\/ml of retrovirus containing rat HO-1 antisense (LSN-RHO-1-AS) or control retrovirus (LXSN). After 3 mo, a 0.25-mm U-shaped silver clip was placed around the left renal artery. Animals were killed 3 wk later. Clipping the renal artery in LSN-RHO-1-AS rats did not result in increased HO-1 expression. In contrast to LXSN animals, 2K1C LSN-RHO-1-AS rats showed increased expression of cyclooxygenase 2 (COX-2) and higher 3-nitrotyrosine (3-NT) content as well as increased expression of the proapoptotic protein Apaf-1 and caspase-3 activity. Clipping the renal artery in LXSN rats resulted in increased expression of the antiapoptotic proteins Bcl-2 and Bcl-xl, while clipping the renal artery in LSN-RHO-1-AS rats did not change Bcl-2 levels and decreased the levels of Bcl-xl. Treatment of LSN-RHO-1-AS rats with cobalt protoporphyrin resulted in induction of renal HO-1, which was accompanied by decreases in blood pressure, COX-2, 3-NT, and caspase-3 activity, and increased expression of anti-apoptotic molecules (Bcl-2, Bcl-xl, Akt and p-Akt) in the clipped kidneys. These findings underscore the prominent role of HO-1 in counteracting apoptosis in this 2K1C renovascular hypertension model.<\/jats:p>","DOI":"10.1152\/ajprenal.00261.2006","type":"journal-article","created":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T08:32:02Z","timestamp":1156926722000},"page":"F148-F157","source":"Crossref","is-referenced-by-count":33,"title":["Genetic suppression of HO-1 exacerbates renal damage: reversed by an increase in the antiapoptotic signaling pathway"],"prefix":"10.1152","volume":"292","author":[{"given":"Rafal","family":"Olszanecki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Rezzani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Omura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Stec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Rodella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fady T.","family":"Botros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvin I.","family":"Goodman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Drummond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nader G.","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1159\/000154819"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.03.010"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000091828.36599.34"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.414"},{"key":"R5","unstructured":"Agarwal A, Nick HS.Renal response to tissue injury: lessons from heme oxygenase-1 gene ablation and expression.J Am Soc Nephrol11: 965\u2013973, 2000."},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4839"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200204150-00011"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9285"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00080.x"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1161\/hy0202.103420"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00745.x"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5482.1150"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.7.1015"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0750304.x"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2005.00826.x"},{"key":"R16","doi-asserted-by":"crossref","unstructured":"Datta PK, Koukouritaki SB, Hopp KA, Lianos EA.Heme oxygenase-1 induction attenuates inducible nitric oxide synthase expression and proteinuria in glomerulonephritis.J Am Soc Nephrol12: 2540\u20132550, 1999.","DOI":"10.1681\/ASN.V10122540"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2445"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.68.1.383"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1007\/s003950170052"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/11072"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81883-8"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00686.x"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1101\/gad.889901"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1309"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000315"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.3.893-901.2001"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.04.161"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.13.7557017"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.37.2.541"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.2174\/092986706775527956"},{"key":"R31","unstructured":"Maines MD, Mayer RD, Ewing JF.Induction of kidney heme oxygenase-1 (HSP32) mRNA and protein by ischemia reperfusion: possible role of heme as both promoter of tissue damage and regulator of HSP32.J Pharmacol Exp Ther264: 457\u2013462, 1993."},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115847"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00471.x"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.20.10925"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.01.086"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.211399398"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(03)00118-4"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20005"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.278.5.F726"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00297.2003"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2004.6.841"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mn.7800141"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.8.2360"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1038\/70237"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01088.2004"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1177\/039139880402701203"},{"key":"R47","doi-asserted-by":"crossref","unstructured":"Wagener FA, da Silva JL, Farley T, de Witte T, Kappas A, Abraham NG.Differential effects of heme oxygenase isoforms on heme mediation of endothelial intracellular adhesion molecule 1 expression.J Pharmacol Exp Ther291: 416\u2013423, 1999.","DOI":"10.1016\/S0022-3565(24)35116-X"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00897.x"},{"key":"R49","unstructured":"Wang X.The expanding role of mitochondria in apoptosis.Genes Dev15: 2922\u20132933, 2001."},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000052945.84627.8F"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1161\/hh1001.091521"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.0000126287.62060.e6"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81733-X"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.17.11549"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00261.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T18:06:34Z","timestamp":1736532394000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00261.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1152\/ajprenal.00261.2006"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00261.2006","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}