{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:06:04Z","timestamp":1758819964723},"reference-count":28,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2007,6]]},"abstract":" Furosemide administration stimulates distal acidification. This has been attributed to the increased lumen-negative voltage in the distal nephron, but the aspect of regulatory mechanisms of H+<\/jats:sup>-ATPase has not been clear. The purpose of this study is to investigate whether chronic administration of diuretics alters the expression of H+<\/jats:sup>-ATPase and whether electrogenic Na+<\/jats:sup> reabsorption is involved in this process. A 7-day infusion of furosemide or hydrochlorothiazide (HCTZ) lowered urine pH significantly. However, this effect of furosemide-induced distal acidification was not changed with amiloride-blocking electrogenic Na+<\/jats:sup> reabsorption. On immunoblotting, a polyclonal antibody against the H+<\/jats:sup>-ATPase B1 subunit recognized a specific \u223c56-kDa band in membrane fractions from the kidney. The protein abundance of H+<\/jats:sup>-ATPase was significantly increased by furosemide and HCTZ infusion in both the cortex and outer medulla. Furosemide plus amiloride administration also increased the H+<\/jats:sup>-ATPase protein abundance significantly. However, no definite subcellular redistribution of H+<\/jats:sup>-ATPase was observed by furosemide \u00b1 amiloride infusion with immunohistochemistry. Chronic furosemide \u00b1 amiloride administration induced a translocation of pendrin to the apical membrane, while total protein abundance was not increased. The mRNA expression of H+<\/jats:sup>-ATPase was not altered by furosemide \u00b1 amiloride infusion. We conclude that chronic administration of diuretics enhances distal acidification by increasing the abundance of H+<\/jats:sup>-ATPase irrespective of electrogenic Na+<\/jats:sup> reabsorption. This upregulation of H+<\/jats:sup>-ATPase in the intercalated cells may be the result of tubular hypertrophy by diuretics. <\/jats:p>","DOI":"10.1152\/ajprenal.00270.2006","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T03:59:52Z","timestamp":1172030392000},"page":"F1701-F1709","source":"Crossref","is-referenced-by-count":18,"title":["Chronic furosemide or hydrochlorothiazide administration increases H+<\/sup>-ATPase B1 subunit abundance in rat kidney"],"prefix":"10.1152","volume":"292","author":[{"given":"Ki Young","family":"Na","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gheun-Ho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwon Wook","family":"Joo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay Wook","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Ryoun","family":"Jang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Kyu","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Un Sil","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seoung-Wan","family":"Chae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Knepper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Suk","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1986.220"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00340.2006"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1977.232.5.F397"},{"key":"R4","unstructured":"Costanzo LS. 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