{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:10:52Z","timestamp":1778274652278,"version":"3.51.4"},"reference-count":107,"publisher":"American Physiological Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:p>The renal proximal tubule (PT) is a major site for maintaining whole body pH homeostasis and is responsible for reabsorbing \u223c80% of filtered HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>, the major plasma buffer, into the blood. The PT adapts its rate of HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>reabsorption ( J<jats:sub>HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup><\/jats:sub>) in response to acute acid-base disturbances. Our laboratory previously showed that single isolated perfused PTs adapt J<jats:sub>HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup><\/jats:sub>in response to isolated changes in basolateral (i.e., blood side) CO<jats:sub>2<\/jats:sub>and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>concentrations but, surprisingly, not to pH. The response to CO<jats:sub>2<\/jats:sub>concentration can be blocked by the ErbB family tyrosine kinase inhibitor PD-168393. In the present study, we exposed enriched rabbit PT suspensions to five acute acid-base disturbances for 5 and 20 min using a panel of phosphotyrosine (pY)-specific antibodies to determine the influence of each disturbance on pan-pY, ErbB1-specific pY (four sites), and ErbB2-specific pY (two sites). We found that each acid-base treatment generated a distinct temporal pY pattern. For example, the summated responses of the individual ErbB1\/2-pY sites to each disturbance showed that metabolic acidosis (normal CO<jats:sub>2<\/jats:sub>concentration and reduced HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>concentration) produced a transient summated pY decrease (5 vs. 20 min), whereas metabolic alkalosis produced a transient increase. Respiratory acidosis (normal HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>concentration and elevated CO<jats:sub>2<\/jats:sub>concentration) had little effect on summated pY at 5 min but produced an elevation at 20 min, whereas respiratory alkalosis produced a reduction at 20 min. Our data show that ErbB1 and ErbB2 in the PT respond to acute acid-base disturbances, consistent with the hypothesis that they are part of the signaling cascade.<\/jats:p>","DOI":"10.1152\/ajprenal.00307.2013","type":"journal-article","created":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T04:35:32Z","timestamp":1381984532000},"page":"F1747-F1764","source":"Crossref","is-referenced-by-count":9,"title":["Effect of acute acid-base disturbances on ErbB1\/2 tyrosine phosphorylation in rabbit renal proximal tubules"],"prefix":"10.1152","volume":"305","author":[{"given":"Lara A.","family":"Skelton","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter F.","family":"Boron","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, 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