{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T02:02:28Z","timestamp":1793325748283,"version":"4.5.0"},"reference-count":24,"publisher":"American Physiological Society","issue":"3","funder":[{"name":"National Natural Science Foundation of China General Projects","award":["31371172"],"award-info":[{"award-number":["31371172"]}]},{"name":"Shanghai International Science and Technology Cooperation Fund Project","award":["0954070200"],"award-info":[{"award-number":["0954070200"]}]},{"name":"System redesign and demonstration for early detection, diagnosis and treatment of chronic kidney disease in Shanghai","award":["GWIV-18"],"award-info":[{"award-number":["GWIV-18"]}]},{"name":"The National Key Research and Development Program of China","award":["2016YFC0901500"],"award-info":[{"award-number":["2016YFC0901500"]}]},{"name":"National Science & Technology Pillar Program","award":["2013BAI09B04"],"award-info":[{"award-number":["2013BAI09B04"]}]},{"name":"National Science Foundation for Young Scientists of China","award":["81400687"],"award-info":[{"award-number":["81400687"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2018,9,1]]},"abstract":"<jats:p> The aim of our current study was to investigate the long-term effect and the mechanism of triptolide in an adult nonorthologous rat model of polycystic kidney disease (PKD). Male wild-type (+\/+) and Cy\/+ cystic Han:SPRD rats were treated with vehicle or triptolide from 4 to 16 wk of age. Rats were killed at 16 wk of age for blood, urine, and organ collection. Human-derived WT9\u201312 PKD cells were treated with triptolide with or without IL-6 pretreatment. Cell proliferation, apoptosis, and cytotoxicity were determined. Western blotting and immunohistochemistry analysis were performed to evaluate the activation of IL-6-JAK2-STAT3 pathway. Renal function was protected by 12 wk of triptolide treatment in cystic Han:SPRD rats as shown by reduced blood urea nitrogen, serum creatinine, and proteinuria levels. Cyst and kidney growth were also retarded by triptolide treatment in Cy\/+ rats. We further found that the proliferation index was reduced by triptolide in cystic rats, which was correlated with the reduced expression of IL-6\/IL-6 receptor, decreased phosphorylation of JAK2-STAT3, and increased expression of suppressor of cytokine signaling 3 (SOCS3). The inhibitory effect of triptolide was further studied in WT9\u201312 cells. Triptolide inhibited cell proliferation and the activation of JAK2-STAT3 pathway in PKD cells, but it increased the expression of SOCS3. Pretreatment with IL-6 attenuated the inhibitory effect of triptolide on STAT3 phosphorylation. Our study revealed a long-term beneficial effect of triptolide in PKD that was probably through inhibition of the JAK2-STAT3 pathway. <\/jats:p>","DOI":"10.1152\/ajprenal.00329.2017","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T09:08:29Z","timestamp":1520413709000},"page":"F479-F486","source":"Crossref","is-referenced-by-count":27,"title":["Triptolide delays disease progression in an adult rat model of polycystic kidney disease through the JAK2-STAT3 pathway"],"prefix":"10.1152","volume":"315","author":[{"given":"Ying","family":"Jing","sequence":"first","affiliation":[{"name":"Kidney Institute, Department of Nephrology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China"},{"name":"Department of Blood Purification, General Hospital of Jinan Military Command of People\u2019s Liberation Army, Jinan, 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