{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:30:09Z","timestamp":1774564209689,"version":"3.50.1"},"reference-count":33,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>We studied the effects of two mutations of the extracellular loop of the \u03b1-subunit of the (ENaC) on amiloride-sensitive current in Xenopus laevis oocytes and the inhibition of this current by 3-morpholinosydnonimine (SIN-1). Injection of oocytes with wild-type (wt) \u03b1-,\u03b2-,\u03b3-rENaC cRNA (8.3 ng\/subunit) resulted 48\u201372 h later in inward Na<jats:sup>+<\/jats:sup>currents (\u22125.5 \u00b1 0.8 \u03bcA; means \u00b1 SE at \u2212100 mV; n = 21), which were completely inhibited by amiloride. Oocytes injected with either \u03b1<jats:sub>Y279A<\/jats:sub>- or \u03b1<jats:sub>Y283A<\/jats:sub>- and \u03b2-,\u03b3-rENaC cRNAs had significantly lower Na<jats:sup>+<\/jats:sup>currents. Furthermore, \u03b1<jats:sub>Y279A<\/jats:sub>-,\u03b2-,\u03b3-rENaC-injected oocytes had a higher K<jats:sub>i<\/jats:sub>for amiloride (0.54 \u00b1 0.97 vs. 0.10 \u00b1 0.04 \u03bcM; P &lt; 0.01). Exposure of oocytes to SIN-1 (1 mM) for 5 min decreased both total Na<jats:sup>+<\/jats:sup>and amiloride-sensitive currents across wt and \u03b1<jats:sub>Y279A<\/jats:sub>- but not \u03b1<jats:sub>Y283A<\/jats:sub>-,\u03b2-,\u03b3-rENaC. Furthermore, exposure to SIN-1 increased the K<jats:sub>i<\/jats:sub>for amiloride across wt but not \u03b1<jats:sub>Y279A<\/jats:sub>-,\u03b2-,\u03b3-rENaC-injected oocytes. These data indicate that both tyrosines are important for proper ENaC function and their oxidative modifications contribute to altered ENaC function.<\/jats:p>","DOI":"10.1152\/ajprenal.00352.2003","type":"journal-article","created":{"date-parts":[[2004,2,24]],"date-time":"2004-02-24T01:47:26Z","timestamp":1077587246000},"page":"F1202-F1208","source":"Crossref","is-referenced-by-count":21,"title":["Mutations in the extracellular loop of \u03b1-rENaC alter sensitivity to amiloride and reactive species"],"prefix":"10.1152","volume":"286","author":[{"given":"Lan","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine M.","family":"Fuller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas 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