{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:58:35Z","timestamp":1761155915405},"reference-count":20,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>Previous reports have shown a stimulatory effect of vasopressin (VP) on Na-K-ATPase and rBSC-1 expression and activity. Whether these VP-dependent mechanisms are operating in vivo in physiological conditions as well as in chronic renal failure (CRF) has been less well studied. We measured ATPase expression and activity and rBSC-1 expression in the outer medulla of controls and moderate CRF rats both before and under in vivo inhibition of VP by OPC-31260, a selective V<jats:sub>2<\/jats:sub>-receptor antagonist. OPC-31260 decreased Na-K-ATPase activity from 11.2 \u00b1 1.5 to 3.7 \u00b1 0.8 in controls ( P &lt; 0.05) and from 19.0 \u00b1 0.8 to 2.9 \u00b1 0.5 \u03bcmol P<jats:sub>i<\/jats:sub>\u00b7 mg protein<jats:sup>\u22121<\/jats:sup>\u00b7 h<jats:sup>\u22121<\/jats:sup>in moderate CRF rats ( P &lt; 0.05). CRF was associated with a significant increase in Na-K-ATPase activity ( P &lt; 0.05). Similarly, CRF was also associated with a significant increase in Na-K-ATPase expression to 164.4 \u00b1 21.5% compared with controls ( P &lt; 0.05), and OPC-31260 decreased Na-K-ATPase expression in both controls and CRF rats to 57.6 \u00b1 9.5 and 105.3 \u00b1 10.9%, respectively ( P &lt; 0.05). On the other hand, OPC-31260 decreased rBSC-I expression in both controls and CRF rats to 60.8 \u00b1 6.5 and 30.0 \u00b1 6.9%, respectively ( P &lt; 0.05), and was not influenced by CRF (95.7 \u00b1 5.2%). We conclude that 1) endogenous VP modulated Na-K-ATPase and rBSC-1 in both controls and CRF; and 2) CRF was associated with increased activity and expression of the Na-K-ATPase in the outer medulla, in contrast to the unaltered expression of the rBSC-1. The data suggest that endogenous VP could participate in the regulation of electrolyte transport at the level of the outer medulla.<\/jats:p>","DOI":"10.1152\/ajprenal.00354.2000","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:33:46Z","timestamp":1425414826000},"page":"F265-F270","source":"Crossref","is-referenced-by-count":15,"title":["Endogenous vasopressin regulates Na-K-ATPase and Na<sup>+<\/sup>-K<sup>+<\/sup>-Cl<sup>\u2212<\/sup>cotransporter<i>rbsc-1<\/i>in rat outer medulla"],"prefix":"10.1152","volume":"282","author":[{"given":"Claudia A.","family":"Bertuccio","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones M\u00e9dicas Alfredo Lanari, University of Buenos Aires, 1427 Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando R.","family":"Ibarra","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones M\u00e9dicas Alfredo Lanari, University of Buenos Aires, 1427 Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge E.","family":"Toledo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones M\u00e9dicas Alfredo Lanari, University of Buenos Aires, 1427 Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elvira E.","family":"Arrizurieta","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones M\u00e9dicas Alfredo Lanari, University of Buenos Aires, 1427 Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo S.","family":"Martin","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones M\u00e9dicas Alfredo Lanari, University of Buenos Aires, 1427 Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","first-page":"339","volume":"55","author":"Bertuccio C","year":"1995","journal-title":"Medicina (B. Aires)"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201X.1998.00411.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1994.90"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1979.236.5.F454"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1270213"},{"key":"B6","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1681\/ASN.V122207","volume":"12","author":"Ecelbarger CA","year":"2001","journal-title":"J Am Soc Nephrol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.271.3.F619"},{"key":"B8","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/j.1748-1716.1992.tb09284.x","volume":"144","author":"Fryckstedt J","year":"1992","journal-title":"Acta Physiol Scand"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1994.266.4.F536"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.270.5.F862"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1980.239.3.F281"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.6"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.1.F96"},{"key":"B15","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1681\/ASN.V103628","volume":"10","author":"Knepper MA","year":"1999","journal-title":"J Am Soc Nephrol"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.277.2.F257"},{"key":"B17","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1172\/JCI649"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1989.256.3.F490"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1007\/BF00582563"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb09058.x"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00354.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T09:33:48Z","timestamp":1651397628000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00354.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2,1]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,2,1]]}},"alternative-id":["10.1152\/ajprenal.00354.2000"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00354.2000","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2,1]]}}}