{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:44:24Z","timestamp":1790837064543,"version":"4.1.0"},"reference-count":63,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["2014R1A5A2009242"],"award-info":[{"award-number":["2014R1A5A2009242"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["2016R1A2B4009365"],"award-info":[{"award-number":["2016R1A2B4009365"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2018,3,1]]},"abstract":"<jats:p>Mineralocorticoids trigger a profibrotic process in the kidney. In mouse cortical collecting duct cells, the present study addressed two main questions: 1) what are microRNAs (miRNAs) and their target genes that are changed by aldosterone? and 2) what do miRNAs, in response to aldosterone, regulate regarding signaling pathways related to fibrosis? A microarray chip assay was done in cells in the absence or presence of aldosterone treatment (10<jats:sup>\u22126<\/jats:sup>M; 3 days). The candidate miRNAs were identified by the criteria of &gt;30% of fold change among the significantly changed miRNAs ( P &lt; 0.05). Twenty-nine miRNAs were upregulated (&gt;1.3-fold), and 27 miRNAs were downregulated (&lt;0.7-fold). Putative target genes of identified miRNAs were associated with 74 Kyoto Encyclopedia of Genes and Genomes pathways. Among them, the wingless-related integration site (Wnt) signaling pathway was highly ranked, where 15 mature miRNAs were observed. These miRNAs were further analyzed by real-time quantitative PCR, and among them, miR-130b-3p, miR-34c-5p, and miR-146a-5p were selected. Through the identification of putative target genes of these three miRNAs, mRNA and protein expression of the Ca<jats:sup>2+<\/jats:sup>\/calmodulin-dependent protein kinase type II \u03b2-chain ( Camk2b) gene (a target gene of miR-34c-5p) were found to be increased significantly in aldosterone-treated cells, where fibronectin (FN) and \u03b1-smooth muscle actin were induced. When CaMKII\u03b2 small interfering RNA or the miR-34c-5p mimic was transfected, aldosterone-induced FN expression was significantly attenuated, along with reduced CaMKII\u03b2 protein expression. A luciferase reporter assay revealed a decrease of CaMKII\u03b2 translation in cells transfected with miRNA mimics of miR-34c-5p. In conclusion, aldosterone-induced downregulation of miR-34c-5p in the Wnt signaling pathway and a consequent increase of CaMKII\u03b2 expression are likely to be involved in aldosterone-induced fibrosis.<\/jats:p>","DOI":"10.1152\/ajprenal.00358.2017","type":"journal-article","created":{"date-parts":[[2017,10,25]],"date-time":"2017-10-25T15:06:11Z","timestamp":1508943971000},"page":"F329-F342","source":"Crossref","is-referenced-by-count":40,"title":["miR-34c-5p and CaMKII are involved in aldosterone-induced fibrosis in kidney collecting duct cells"],"prefix":"10.1152","volume":"314","author":[{"given":"Eui-Jung","family":"Park","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea"},{"name":"BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Taegu, 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