{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:46:44Z","timestamp":1778276804898,"version":"3.51.4"},"reference-count":46,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p>Pendrin ( Slc26a4) localizes to type B and non-A, non-B intercalated cells in the distal convoluted tubule, the connecting tubule, and the cortical collecting duct (CCD), where it mediates apical Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>exchange. The purpose of this study was to determine whether angiotensin II increases transepithelial net chloride transport, J<jats:sub>Cl<\/jats:sub>, in mouse CCD through a pendrin-dependent mechanism. J<jats:sub>Cl<\/jats:sub>and transepithelial voltage, V<jats:sub>T<\/jats:sub>, were measured in CCDs perfused in vitro from wild-type and Slc26a4 null mice ingesting a NaCl-replete diet or a NaCl-replete diet and furosemide. In CCDs from wild-type mice ingesting a NaCl-replete diet, V<jats:sub>T<\/jats:sub>and J<jats:sub>Cl<\/jats:sub>were not different from zero either in the presence or absence of angiotensin II (10<jats:sup>\u22128<\/jats:sup>M) in the bath. Thus further experiments employed mice given the high-NaCl diet and furosemide to upregulate renal pendrin expression. CCDs from furosemide-treated wild-type mice had a lumen-negative V<jats:sub>T<\/jats:sub>and absorbed Cl<jats:sup>\u2212<\/jats:sup>. With angiotensin II in the bath, Cl<jats:sup>\u2212<\/jats:sup>absorption doubled although V<jats:sub>T<\/jats:sub>did not become more lumen negative. In contrast, in CCDs from furosemide-treated Slc26a4 null mice, Cl<jats:sup>\u2212<\/jats:sup>secretion and a V<jats:sub>T<\/jats:sub>of \u223c0 were observed, neither of which changed with angiotensin II application. Inhibiting ENaC with benzamil abolished V<jats:sub>T<\/jats:sub>although J<jats:sub>Cl<\/jats:sub>fell only \u223c50%. Thus substantial Cl<jats:sup>\u2212<\/jats:sup>absorption is observed in the absence of an electromotive force. Attenuating apical anion exchange with the peritubular application of the H<jats:sup>+<\/jats:sup>-ATPase inhibitor bafilomycin abolished benzamil-insensitive Cl<jats:sup>\u2212<\/jats:sup>absorption. In conclusion, angiotensin II increases transcellular Cl<jats:sup>\u2212<\/jats:sup>absorption in the CCD through a pendrin- and H<jats:sup>+<\/jats:sup>-ATPase-dependent process.<\/jats:p>","DOI":"10.1152\/ajprenal.00361.2006","type":"journal-article","created":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T04:45:01Z","timestamp":1162356301000},"page":"F914-F920","source":"Crossref","is-referenced-by-count":106,"title":["Angiotensin II increases chloride absorption in the cortical collecting duct in mice through a pendrin-dependent mechanism"],"prefix":"10.1152","volume":"292","author":[{"given":"Vladim\u00edr","family":"Pech","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan M.","family":"Weinstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine A.","family":"Everett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Truyen D.","family":"Pham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Wall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Bouby N, Hus-Citharel A, Marchetti J, Bankir L, Corvol P, Llorens-Cortes C.Expression of type 1 angiotensin II receptor subtypes and angiotensin II-induced calcium mobilization along the rat nephron.J Am Soc Nephrol8: 1658\u20131667, 1997.","DOI":"10.1681\/ASN.V8111658"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Drose S, Altendorf K.Bafilomycins and concanamycins as inhibitors of V-ATPases and P-ATPases.J Exp Biol200: 1\u20138, 1997.","DOI":"10.1242\/jeb.200.1.1"},{"key":"R3","unstructured":"Emmons C.H+extrusion in mouse cortical collecting duct (CCD) intercalated cells (ICs) (Abstract).J Am Soc Nephrol8: 5A, 1997."},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.2.153"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00239.x"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1985.249.2.F205"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.20.7917"},{"key":"R8","unstructured":"Hall JE, Brands MW.The renin-angiotensin-aldosterone systems: renal mechanisms circulatory homeostasis. 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