{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:20:05Z","timestamp":1761765605669,"version":"3.40.4"},"reference-count":45,"publisher":"American Physiological Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:p>We investigated the effects of partial bladder outlet obstruction (BOO) on the function and gene expression of 5-hydroxytryptamine (5-HT) receptor subtypes in rat bladder. Isometric contractions of the isolated bladders from sham-operated control and BOO rats were examined. The contractile responses to 5-HT were significantly increased in BOO rat bladder strips, while the responses to KCl, carbachol, or phenylephrine were not different from the control. The 5-HT-induced hypercontraction in BOO rat bladder strips was inhibited by ketanserin, a 5-HT<jats:sub>2A<\/jats:sub>receptor antagonist. The contractile responses to 5-HT in bladder strips were not affected by urothelium removal from the intact bladder. The gene expression of 5-HT receptor subtypes in the bladders was analyzed by RT-PCR. The mRNA expression of the 5-HT<jats:sub>2A<\/jats:sub>, 5-HT<jats:sub>2B<\/jats:sub>, 5-HT<jats:sub>2C<\/jats:sub>, 5-HT<jats:sub>4<\/jats:sub>, and 5-HT<jats:sub>7<\/jats:sub>receptors was detected in both the control and BOO rat bladders. Quantitative RT-PCR analysis showed there was a significant increase of 5-HT<jats:sub>2A<\/jats:sub>receptor mRNA in the BOO rat bladder compared with the control bladder. On the other hand, the gene expression of the 5-HT<jats:sub>4<\/jats:sub>receptor was not changed in the BOO rat bladder. These results suggest that the increased contractile responses to 5-HT in BOO rat bladder may be partly caused by 5-HT<jats:sub>2A<\/jats:sub>receptor upregulation in the detrusor smooth muscles.<\/jats:p>","DOI":"10.1152\/ajprenal.00365.2012","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T10:25:23Z","timestamp":1359023123000},"page":"F1020-F1027","source":"Crossref","is-referenced-by-count":21,"title":["5-Hydroxytryptamine-induced bladder hyperactivity via the 5-HT<sub>2A<\/sub>receptor in partial bladder outlet obstruction in rats"],"prefix":"10.1152","volume":"304","author":[{"given":"Takumi","family":"Sakai","sequence":"first","affiliation":[{"name":"Laboratory for Development Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, Mifuku, Izunokuni-shi, Shizuoka, 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