{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:33:29Z","timestamp":1783571609211,"version":"3.55.0"},"reference-count":40,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:p> The roles of epithelial sodium channel (ENaC) subunits (\u03b1, \u03b2, and \u03b3) in the impaired renal reabsorption of sodium and water were examined in rat models with bilateral (BUO) or unilateral ureteral obstruction (UUO) for 24 h or with BUO followed by release of obstruction and 3 days of observation (BUO-3dR). In BUO rats, plasma osmolality was increased dramatically, whereas plasma sodium concentration was decreased. Immunoblotting revealed a significantly decreased expression of \u03b1-ENaC (57 \u00b1 7%), \u03b2-ENaC (19 \u00b1 5%), and \u03b3-ENaC (51 \u00b1 10%) as well as 11\u03b2-hydroxysteroid dehydrogenase type 2 (11\u03b2-HSD2) in the cortex and outer medulla (C+OM) compared with sham-operated controls. This was confirmed by immunohistochemistry. BUO-3dR was associated with polyuria and impaired renal sodium handling. The protein abundance and the apical labeling of \u03b1-ENaC were significantly increased, whereas \u03b2- and \u03b3-ENaC as well as 11\u03b2-HSD2 expression remained decreased. In UUO rats, expression of \u03b1- and \u03b2-ENaC and 11\u03b2-HSD2 decreased in the C+OM in the obstructed kidney. In contrast, the abundance and the apical labeling of \u03b1-ENaC in the nonobstructed kidneys were markedly increased, suggesting compensatory upregulation in this kidney. In conclusion, \u03b1-, \u03b2-, and \u03b3-ENaC expression levels are downregulated in the obstructed kidney. The expression and apical labeling of \u03b1-ENaC were increased in BUO-3dR rats and in the nonobstructed kidneys from UUO rats. These results suggest that altered expression of \u03b1-, \u03b2-, and \u03b3-ENaC may contribute to impaired renal sodium and water handling in response to ureteral obstruction. <\/jats:p>","DOI":"10.1152\/ajprenal.00372.2006","type":"journal-article","created":{"date-parts":[[2007,5,3]],"date-time":"2007-05-03T15:58:56Z","timestamp":1178207936000},"page":"F333-F341","source":"Crossref","is-referenced-by-count":19,"title":["Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction"],"prefix":"10.1152","volume":"293","author":[{"given":"Chunling","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke","family":"Norregaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Knepper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen","family":"Fr\u00f8ki\u00e6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108728"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/367463a0"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1991.tb15277.x"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gag091"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.1.F46"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1993.265.6.F875"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.2.F181"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1997.273.2.F213"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.270.4.F657"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1126\/science.2845584"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199715020-00008"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.6.F1093"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1038\/ng0995-76"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1038\/ng0396-325"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116173"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107127"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1990.258.5.F1320"},{"key":"R18","unstructured":"Klahr S. 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