{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T10:54:09Z","timestamp":1772794449328,"version":"3.50.1"},"reference-count":22,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:p>Corticosteroid hormone-induced factor (CHIF) is a short epithelial-specific protein that is independently induced by aldosterone and a high-K<jats:sup>+<\/jats:sup>diet. It is a member of the FXYD family of single-span transmembrane proteins that include phospholemman, Mat-8, and the \u03b3-subunit of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase. A number of studies have suggested that these proteins are involved in the regulation of ion transport and, in particular, functionally interact with the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase. The present study describes the characterization, targeted disruption, and phenotypic analysis of the mouse CHIF gene. The CHIF knockout mice are viable and not distinguishable from wild-type littermates under normal conditions. Under K<jats:sup>+<\/jats:sup>loading, they have a twofold higher urine volume and an increased glomerular filtration rate. Similar but smaller effects are observed in mice fed a low-Na<jats:sup>+<\/jats:sup>diet. Treating K<jats:sup>+<\/jats:sup>-loaded mice for 10 days with furosemide resulted in lethality in the knockout mice (17 of 39) but not in the wild-type group (1 of 39). The data are consistent with an effect of CHIF on the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase that is specific to the outer and inner medullary duct, its major expression site.<\/jats:p>","DOI":"10.1152\/ajprenal.00376.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:35:50Z","timestamp":1425414950000},"page":"F569-F577","source":"Crossref","is-referenced-by-count":23,"title":["Generation and phenotypic analysis of CHIF knockout mice"],"prefix":"10.1152","volume":"283","author":[{"given":"Roman","family":"Aizman","sequence":"first","affiliation":[{"name":"Departments of Biological Chemistry and"},{"name":"Department of Anatomy, Physiology and Health Education, Novosibirsk State Pedagogical University, Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol","family":"Asher","sequence":"additional","affiliation":[{"name":"Departments of Biological Chemistry and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"F\u00fczesi","sequence":"additional","affiliation":[{"name":"Departments of Biological Chemistry and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hedva","family":"Latter","sequence":"additional","affiliation":[{"name":"Departments of Biological Chemistry and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Lonai","sequence":"additional","affiliation":[{"name":"Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J. 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