{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T13:10:23Z","timestamp":1779196223657,"version":"3.51.4"},"reference-count":39,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p>Detrusor smooth muscle (DSM) undergoes hypertrophy after partial bladder outlet obstruction (PBOO) in male rabbits, as it does in men with PBOO induced by benign prostatic hyperplasia. Despite detrusor hypertrophy, some bladders are severely dysfunctional (decompensated). In this study, the rabbit model for PBOO was used to determine the biochemical regulation of the contractile apparatus and force maintenance by the detrusor from decompensated bladders (DB). Bladders from sham-operated rabbits served as a control. On stimulation with 125 mM KCl, the DSM from sham-operated (SB) rabbits showed phasic contractions, whereas the detrusor from DB was tonic, exhibiting slow development of force, a longer duration of force maintenance, and slow relaxation. The Rho kinase (ROK) inhibitor Y-27632 enhanced the relaxation of precontracted DSM strips from DB. The enhancement of relaxation of the KCl-induced contraction of DB by Y-27632 was associated with dephosphorylation of myosin light chain (MLC<jats:sub>20<\/jats:sub>). The DSM extract from DB showed low phosphatase activity compared with that from SB. The DB also showed more Ca<jats:sup>2+<\/jats:sup>-independent MLC<jats:sub>20<\/jats:sub>phosphorylation, which was partially inhibited by Y-27632. RT-PCR and Western blotting revealed similar expression levels of MLC kinase and ROK-\u03b1 in SB and DB, but ROK-\u03b2 was overexpressed in DB. These results suggest that the ROK-mediated pathway is partly responsible for the high degree of force maintenance and slow relaxation in the detrusor from DB.<\/jats:p>","DOI":"10.1152\/ajprenal.00378.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:39:33Z","timestamp":1425415173000},"page":"F990-F997","source":"Crossref","is-referenced-by-count":124,"title":["Obstruction-induced changes in urinary bladder smooth muscle contractility: a role for Rho kinase"],"prefix":"10.1152","volume":"285","author":[{"given":"Wu","family":"Bing","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A.","family":"Hypolite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"DiSanto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Zderic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lester","family":"Rolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Wein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Chacko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.004421"},{"key":"REF2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.34.20246"},{"key":"REF3","unstructured":"Butler TMand Siegman MJ.Chemical energetics of contraction in mammalian smooth muscle.Fed Proc41: 204-208, 1982."},{"key":"REF4","doi-asserted-by":"publisher","DOI":"10.1021\/bi00507a005"},{"key":"REF5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.1.129"},{"key":"REF6","doi-asserted-by":"publisher","DOI":"10.1016\/S1569-2590(00)08004-6"},{"key":"REF7","doi-asserted-by":"publisher","DOI":"10.1038\/83258"},{"key":"REF8","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011634200"},{"key":"REF9","doi-asserted-by":"publisher","DOI":"10.1126\/science.6893872"},{"key":"REF10","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.5.C1532"},{"key":"REF11","unstructured":"DiSanto ME, Hypolite JA, Zheng Y, Malkowicz SB, Wein AJ, and Chacko S.Alteration of contractility in hypertrophied urinary bladder smooth muscle is associated with an overexpression of the SM-A and LC17bmyosin isoforms (Abstract).Biophys J76: A13, 1999."},{"key":"REF12","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006917032557"},{"key":"REF13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1980.tb29606.x"},{"key":"REF14","doi-asserted-by":"crossref","unstructured":"Hypolite JA, DiSanto ME, Zheng Y, Chang S, Wein AJ, and Chacko S.Regional variation in myosin isoforms and phosphorylation at the resting tone in urinary bladder smooth muscle.Am J Physiol Cell Physiol280: C254-C264, 2001.","DOI":"10.1152\/ajpcell.2001.280.2.C254"},{"key":"REF15","doi-asserted-by":"crossref","unstructured":"Ishizaki T, Uehata M, Tamechika I, Keel J, Nonomura K, Maekawa M, and Narumiya S.Pharmacological properties of Y-27632, a specific inhibitor of Rho-associated kinases.Mol Pharmacol57: 976-983, 2000.","DOI":"10.1016\/S0026-895X(24)26508-1"},{"key":"REF16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1985.249.3.C238"},{"key":"REF17","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.5.1023"},{"key":"REF18","unstructured":"Kawano Y, Yoshimura T, and Kaibuchi K.Smooth muscle contraction by small GTPase Rho.Nagoya J Med Sci65: 1-8, 2002."},{"key":"REF19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.20.9307"},{"key":"REF20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000857200"},{"key":"REF21","unstructured":"Levin RM, Haugaard N, Levin SS, Buttyan R, Chen M, Monson FC, and Wein AJ.Bladder function in experimental outlet obstruction: pharmacologic responses to alterations in innervation, energetics, calcium mobilization, and genetics. 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