{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:06:19Z","timestamp":1790895979499,"version":"4.1.0"},"reference-count":58,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>AVP resistance of the medullary collecting duct (mCD) in postobstructive uropathy (POU) has been attributed to increased production of PGE<jats:sub>2<\/jats:sub>. P2Y<jats:sub>2<\/jats:sub>receptor activation causes production of PGE<jats:sub>2<\/jats:sub>by the mCD. We hypothesize that increased P2Y<jats:sub>2<\/jats:sub>receptor expression and\/or activity may contribute to the diuresis of POU. Sprague-Dawley rats were subjected to bilateral ureteral obstruction for 24 h followed by release (BUO\/R, n = 17) or sham operation (SHM\/O, n = 15) and euthanized after 1 wk or 12 days. BUO\/R rats developed significant polydipsia, polyuria, urinary concentration defect, and increased urinary PGE<jats:sub>2<\/jats:sub>and decreased aquaporin-2 protein abundance in the inner medulla compared with SHM\/O rats. After BUO\/R, the relative mRNA expression of P2Y<jats:sub>2<\/jats:sub>and P2Y<jats:sub>6<\/jats:sub>receptors was increased by 2.7- and 4.9-fold, respectively, without significant changes in mRNA expression of P2Y<jats:sub>1<\/jats:sub>or P2Y<jats:sub>4<\/jats:sub>receptor. This was associated with a significant 3.5-fold higher protein abundance of the P2Y<jats:sub>2<\/jats:sub>receptor in BUO\/R than SHM\/O rats. When freshly isolated mCD fractions were challenged with different types of nucleotides (ATP\u03b3S, ADP, UTP, or UDP), BUO\/R and SHM\/O rats responded to only ATP\u03b3S and UTP and released PGE<jats:sub>2<\/jats:sub>, consistent with involvement of the P2Y<jats:sub>2<\/jats:sub>, but not P2Y<jats:sub>6<\/jats:sub>, receptor. ATP\u03b3S- or UTP-stimulated increases in PGE<jats:sub>2<\/jats:sub>were much higher in BUO\/R (3.20- and 2.28-fold, respectively, vs. vehicle controls) than SHM\/O (1.68- and 1.30-fold, respectively, vs. vehicle controls) rats. In addition, there were significant 2.4- and 2.1-fold increases in relative mRNA expression of prostanoid EP<jats:sub>1<\/jats:sub>and EP<jats:sub>3<\/jats:sub>receptors, respectively, in the inner medulla of BUO\/R vs. SHM\/O rats. Taken together, these data suggest that increased production of PGE<jats:sub>2<\/jats:sub>by the mCD in POU may be due to increased expression and activity of the P2Y<jats:sub>2<\/jats:sub>receptor. Increased mRNA expression of EP<jats:sub>1<\/jats:sub>and EP<jats:sub>3<\/jats:sub>receptors in POU may also help accentuate PGE<jats:sub>2<\/jats:sub>-induced signaling in the mCD.<\/jats:p>","DOI":"10.1152\/ajprenal.00382.2009","type":"journal-article","created":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T02:37:43Z","timestamp":1260412663000},"page":"F634-F642","source":"Crossref","is-referenced-by-count":20,"title":["Potential involvement of P2Y<sub>2<\/sub>receptor in diuresis of postobstructive uropathy in rats"],"prefix":"10.1152","volume":"298","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"Research and"},{"name":"Departments of 4Internal Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald E.","family":"Kohan","sequence":"additional","affiliation":[{"name":"Nephrology 1Service and"},{"name":"Departments of 4Internal 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