{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:38:16Z","timestamp":1778276296545,"version":"3.51.4"},"reference-count":45,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p>In rat outer medullary collecting duct (OMCD), the mechanism(s) and regulation of H<jats:sup>+<\/jats:sup>secretion are not understood fully. The effect of changes in acid-base balance and the renin-angiotensin system on net H<jats:sup>+<\/jats:sup>secretion was explored. Rats received NaCl, NaHCO<jats:sub>3<\/jats:sub>, NH<jats:sub>4<\/jats:sub>Cl, or nothing in their drinking water for 7 days. Total ammonia and total CO<jats:sub>2<\/jats:sub>( J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>) fluxes were measured in OMCD tubules perfused in vitro from rats in each treatment group. J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>was reduced in tubules from rats drinking NH<jats:sub>4<\/jats:sub>Cl relative to those drinking NaHCO<jats:sub>3<\/jats:sub>. Because NH<jats:sub>4<\/jats:sub>Cl intake increases plasma renin and aldosterone, we asked if upregulation of the renin-angiotensin system reduces net H<jats:sup>+<\/jats:sup>secretion. Deoxycorticosterone pivalate administered in vivo did not affect J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>. However, ANG II given in vivo at 0.1 ng\/min reduced J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>by 35%. To determine if ANG II has a direct effect on acid secretion, J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>was measured with ANG II applied in vitro. ANG II (10<jats:sup>-8<\/jats:sup>M) present in the bath solution reduced J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>by 35%. This ANG II effect was not observed in the presence of the AT<jats:sub>1<\/jats:sub>receptor blocker candesartan. In conclusion, in rat OMCD, J<jats:sub>tCO<jats:sub>2<\/jats:sub><\/jats:sub>is paradoxically reduced with NH<jats:sub>4<\/jats:sub>Cl ingestion. 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