{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:51:26Z","timestamp":1790319086116,"version":"4.1.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:p>Autosomal dominant polycystic kidney disease (ADPKD) arises following mutations of either Pkd1 or Pkd2. The proteins these genes encode, polycystin-1 (PC1) and polycystin-2 (PC2), form a signaling complex using direct intermolecular interactions. Two distinct domains in the C-terminal tail of PC2 have recently been identified, an EF-hand and a coiled-coil domain. Here, we show that the PC2 coiled-coil domain interacts with the C-terminal tail of PC1, but that the PC2 EF-hand domain does not. We measured the K<jats:sub>0.5<\/jats:sub>of the interaction between the C-terminal tails of PC1 and PC2 and showed that the direct interaction of these proteins is abrogated by a PC1 point mutation that was identified in ADPKD patients. Finally, we showed that overexpression of the PC1 C-terminal tail in MDCK cells alters the Ca<jats:sup>2+<\/jats:sup>response, but that overexpression of the PC1 C-terminal tail containing the disease mutation does not. These results allow a more detailed understanding of the mechanism of pathogenic mutations in the cytoplasmic regions of PC1 and PC2.<\/jats:p>","DOI":"10.1152\/ajprenal.00412.2009","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T01:09:05Z","timestamp":1251940145000},"page":"F1310-F1315","source":"Crossref","is-referenced-by-count":34,"title":["Analysis of the cytoplasmic interaction between polycystin-1 and polycystin-2"],"prefix":"10.1152","volume":"297","author":[{"given":"Jozefina","family":"Casuscelli","sequence":"first","affiliation":[{"name":"Departments of 1Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Departments of 1Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"DeGray","sequence":"additional","affiliation":[{"name":"Departments of 1Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward T.","family":"Petri","sequence":"additional","affiliation":[{"name":"Departments of 1Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andjelka","family":"\u0106eli\u0107","sequence":"additional","affiliation":[{"name":"Departments of 1Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Folta-Stogniew","sequence":"additional","affiliation":[{"name":"W. 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