{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T13:55:41Z","timestamp":1789826141576,"version":"4.0.1"},"reference-count":63,"publisher":"American Physiological Society","issue":"2","funder":[{"name":"National Science Foundition for Young Scholars of China to Jing Sun","award":["26020105131538"],"award-info":[{"award-number":["26020105131538"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:p>As an electrophilic nitroalkene fatty acid, nitro-oleic acid (OA-NO<jats:sub>2<\/jats:sub>) exerts multiple biological effects that contribute to anti-inflammation, anti-oxidative stress, and antiapoptosis. However, little is known about the role of OA-NO<jats:sub>2<\/jats:sub>in peritoneal fibrosis. Thus, in the present study, we examined the effects of OA-NO<jats:sub>2<\/jats:sub>on the high glucose (HG)-induced epithelial-mesenchymal transition (EMT) in human peritoneal mesothelial cells (HPMCs) and evaluated the morphological and immunohistochemical changes in a rat model of peritoneal dialysis-related peritoneal fibrosis. In in vitro experiments, we found that HG reduced the expression level of E-cadherin and increased Snail, N-cadherin, and \u03b1-smooth muscle actin expression levels in HPMCs. The above-mentioned changes were attenuated by pretreatment with OA-NO<jats:sub>2<\/jats:sub>. Additionally, OA-NO<jats:sub>2<\/jats:sub>also inhibited HG-induced activation of the transforming growth factor-\u03b2<jats:sub>1<\/jats:sub>\/Smad signaling pathway and NF-\u03baB signaling pathway. Meanwhile, OA-NO<jats:sub>2<\/jats:sub>inhibited HG-induced phosphorylation of Erk and JNK. The results from the in vivo experiments showed that OA-NO<jats:sub>2<\/jats:sub>notably relieved peritoneal fibrosis by decreasing the thickness of the peritoneum; it also inhibited expression of transforming growth factor-\u03b2<jats:sub>1<\/jats:sub>, \u03b1-smooth muscle actin, N-cadherin, and vimentin and enhanced expression of E-cadherin in the peritoneum. Collectively, these results suggest that OA-NO<jats:sub>2<\/jats:sub>inhibits the HG-induced epithelial-mesenchymal transition in HPMCs and attenuates peritoneal dialysis-related peritoneal fibrosis.<\/jats:p>","DOI":"10.1152\/ajprenal.00425.2019","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T09:47:39Z","timestamp":1574675259000},"page":"F457-F467","source":"Crossref","is-referenced-by-count":15,"title":["Nitro-oleic acid inhibits the high glucose-induced epithelial-mesenchymal transition in peritoneal mesothelial cells and attenuates peritoneal fibrosis"],"prefix":"10.1152","volume":"318","author":[{"given":"Wenyan","family":"Su","sequence":"first","affiliation":[{"name":"Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of 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