{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T01:53:32Z","timestamp":1789869212678,"version":"4.0.1"},"reference-count":43,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>A high-salt diet enhances nitric oxide (NO)-induced inhibition of transport in the thick ascending limb (THAL). Long exposures to NO inhibit Na-K-ATPase in cultured cells. We hypothesized that NO inhibits THAL Na-K-ATPase after long exposures and a high-salt diet would augment this effect. Rats drank either tap water or 1% NaCl for 7\u201310 days. Na-K-ATPase activity was assessed by measuring ouabain-sensitive ATP hydrolysis by THAL suspensions. After 2 h, spermine NONOate (SPM; 5 \u03bcM) reduced Na-K-ATPase activity from 0.44 \u00b1 0.03 to 0.30 \u00b1 0.04 nmol P<jats:sub>i<\/jats:sub>\u00b7\u03bcg protein<jats:sup>\u22121<\/jats:sup>\u00b7min<jats:sup>\u22121<\/jats:sup>in THALs from rats on a normal diet ( P &lt; 0.03). Nitroglycerin also reduced Na-K-ATPase activity ( P &lt; 0.04). After 20 min, SPM had no effect (change \u22120.07 \u00b1 0.05 nmol P<jats:sub>i<\/jats:sub>\u00b7\u03bcg protein<jats:sup>\u22121<\/jats:sup>\u00b7min<jats:sup>\u22121<\/jats:sup>). When rats were fed high salt, SPM did not inhibit Na-K-ATPase after 120 min. To investigate whether ONOO<jats:sup>\u2212<\/jats:sup>formed by NO reacting with O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>was involved, we measured O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>production. THALs from rats on normal and high salt produced 35.8 \u00b1 0.3 and 23.7 \u00b1 0.8 nmol O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>\u00b7min<jats:sup>\u22121<\/jats:sup>\u00b7mg protein<jats:sup>\u22121<\/jats:sup>, respectively ( P &lt; 0.01). Because O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>production differed, we studied the effects of the O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>scavenger tempol. In the presence of 50 \u03bcM tempol, SPM did not inhibit Na-K-ATPase after 120 min (0.50 \u00b1 0.05 vs. 0.52 \u00b1 0.07 nmol P<jats:sub>i<\/jats:sub>\u00b7\u03bcg protein<jats:sup>\u22121<\/jats:sup>\u00b7min<jats:sup>\u22121<\/jats:sup>). Propyl gallate, another O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>scavenger, also prevented SPM-induced inhibition of Na-K-ATPase activity. SPM inhibited pump activity in tubules from rats on high salt when O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>levels were increased with xanthine oxidase and hypoxanthine. We concluded that NO inhibits Na-K-ATPase after long exposures when rats are on a normal diet and this inhibition depends on O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>. NO donors do not inhibit Na-K-ATPase in THALs from rats on high salt due to decreased O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>production.<\/jats:p>","DOI":"10.1152\/ajprenal.00427.2003","type":"journal-article","created":{"date-parts":[[2004,5,4]],"date-time":"2004-05-04T00:19:03Z","timestamp":1083629943000},"page":"F224-F230","source":"Crossref","is-referenced-by-count":31,"title":["Inhibition of Na-K-ATPase in thick ascending limbs by NO depends on O<sub>2<\/sub><sup>\u2212<\/sup>and is diminished by a high-salt diet"],"prefix":"10.1152","volume":"287","author":[{"given":"Marisela","family":"Varela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey 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