{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:19:51Z","timestamp":1790079591135,"version":"4.0.1"},"reference-count":34,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"publisher","award":["340600531870"],"award-info":[{"award-number":["340600531870"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:p>Long-term peritoneal dialysis (PD) therapy results in functional and structural alteration of the peritoneal membrane, including epithelial-to-mesenchymal transition (EMT). Interleukin 6 (IL-6) is a local pleiotropic cytokine, hypothesized to play an important role in EMT. This study was designed to investigate the role of IL-6 in EMT and peritoneal membrane dysfunction in long-term PD patients by assessing the level of IL-6 in dialysate and exploring the relationship between IL-6, the related signaling pathway JAK2\/STAT3, and EMT, using in vitro cellular and molecular techniques. Plasma and dialysate levels of IL-6 were significantly higher in PD ultrafiltration failure patients compared with patients without ultrafiltration failure and were negatively correlated with measures of PD adequacy. In vitro IL-6 treatment changed human peritoneal mesothelial cell phenotype from a typical cobblestone-like to a fibroblast-like appearance and increased cell viability. IL-6 treatment increased \u03b1-smooth muscle actin and vascular endothelial growth factor expression but decreased E-cadherin expression. IL-6 treatment activated the JAK\/STAT signaling pathway. However, the JAK2\/STAT3 inhibitor WP1066 prevented IL-6-induced activation of the JAK2\/STAT3 pathway and EMT. We conclude that IL-6 promotes the EMT process, possibly by activating the JAK2\/STAT3 signaling pathway. IL-6 may serve as a novel therapeutic target for preventing EMT, and preservation of the peritoneal membrane may arise from these studies.<\/jats:p>","DOI":"10.1152\/ajprenal.00428.2016","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T13:57:05Z","timestamp":1494511025000},"page":"F310-F318","source":"Crossref","is-referenced-by-count":66,"title":["IL-6 promotes epithelial-to-mesenchymal transition of human peritoneal mesothelial cells possibly through the JAK2\/STAT3 signaling pathway"],"prefix":"10.1152","volume":"313","author":[{"given":"Jing","family":"Xiao","sequence":"first","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Gong","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"},{"name":"Arthritis Research UK Primary Care Centre, Research Institute for Primary Care &amp; Health Sciences, Keele University, Keele, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahai","family":"Yu","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"},{"name":"Arthritis Research UK Primary Care Centre, Research Institute for Primary Care &amp; Health Sciences, Keele University, Keele, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Wang","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanna","family":"Dou","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Liu","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genyang","family":"Cheng","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Lu","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenming","family":"Yuan","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yansheng","family":"Li","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanzheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Nephrology Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006111292"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.2337\/db08-1328"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1159\/000046937"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.07920910"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2369-15-8"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v7.i2.437"},{"key":"B7","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1681\/ASN.V1251046","volume":"12","author":"Davies SJ","year":"2001","journal-title":"J Am Soc Nephrol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.10.022"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002971"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108593"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.10.112"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-014-1029-3"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-015-2648-z"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013030314"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.337"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-014-0091-8"},{"key":"B17","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1177\/089686080002004S02","volume":"20","author":"Mujais S","year":"2000","journal-title":"Perit Dial Int"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201100604"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209281"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfp670"},{"key":"B21","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1177\/089686080602600109","volume":"26","author":"Pecoits-Filho R","year":"2006","journal-title":"Perit Dial Int"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/17.9.1684"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022667"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2015.07.001"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005050566"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.02.018"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.02.005"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.242"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.12.3505"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1159\/000079706"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013e318240541e"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2033-3"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-014-9921-2"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36183"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00428.2016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T13:19:38Z","timestamp":1659014378000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00428.2016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,1]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,8,1]]}},"alternative-id":["10.1152\/ajprenal.00428.2016"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00428.2016","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,1]]}}}