{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:16:39Z","timestamp":1778274999028,"version":"3.51.4"},"reference-count":40,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>Urea plays an important role in the urinary concentrating capacity. Renal inner medullary (IM) urea transporter expression was examined in rats with bilateral (BUO) or unilateral ureteral obstruction (UUO). BUO (24 h) was associated with markedly increased plasma urea (42.4 \u00b1 1.0 vs. 5.2 \u00b1 0.2 mmol\/l) and a significant decease in expression of UT-A1 (28 \u00b1 8% of sham levels), UT-A3 (45 \u00b1 11%), and UT-B (70 \u00b1 8%). Immunocytochemistry confirmed downregulation of UT-A1 and UT-A3 in IM collecting duct and UT-B in the descending vasa recta. Three days after release of BUO, UT-A1, UT-A3, and UT-B remained significantly downregulated (UT-A1: 37 \u00b1 6%; UT-A3: 25 \u00b1 6%; and UT-B: 10 \u00b1 5% of sham levels; P &lt; 0.05) concurrent with a persistent polyuria and a marked reduction in solute-free water reabsorption (115 \u00b1 11 vs. 196 \u00b1 8 \u03bcl\u00b7min<jats:sup>\u22121<\/jats:sup>\u00b7kg<jats:sup>\u22121<\/jats:sup>, P &lt; 0.05). Moreover, 14 days after release of BUO, total UT-A1, UT-A3, and UT-B remained significantly decreased compared with sham-operated controls and urine urea remained reduced (588 \u00b1 43 vs. 1,150 \u00b1 94 mmol\/l). Consistent with increased levels of plasma urea 24 h after onset of UUO (7.4 \u00b1 0.3 vs. 4.8 \u00b1 0.3 mmol\/l), the protein abundance of UT-A1, UT-A3, and UT-B in IM was markedly reduced in the obstructed kidney, which was confirmed by immunocytochemistry. In the nonobstructed kidney, the expression of urea transporters did not change. In conclusion, reduced expression of UT-A1, UT-A3, and UT-B levels in both BUO and UUO rats suggests that urea transporters play important roles in the impaired urinary concentrating capacity in response to urinary tract obstruction.<\/jats:p>","DOI":"10.1152\/ajprenal.00453.2003","type":"journal-article","created":{"date-parts":[[2004,3,2]],"date-time":"2004-03-02T01:46:56Z","timestamp":1078192016000},"page":"F1154-F1162","source":"Crossref","is-referenced-by-count":54,"title":["Altered expression of urea transporters in response to ureteral obstruction"],"prefix":"10.1152","volume":"286","author":[{"given":"Chunling","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet D.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weidong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Knepper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f8ren","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeff M.","family":"Sands","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen","family":"Fr\u00f8ki\u00e6r","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1995.268.6.F1218"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1159\/000016377"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.281.3.F400"},{"key":"R4","doi-asserted-by":"crossref","unstructured":"Bradford AD, Terris JM, Ecelbarger CA, Klein JD, Sands JM, Chou CL, and Knepper MA.97- And 117-kDa forms of collecting duct urea transporter UT-A1 are due to different states of glycosylation.Am J Physiol Renal Physiol281: F133\u2013F143, 2001.","DOI":"10.1152\/ajprenal.2001.281.1.F133"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00264.2001"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1997.273.2.F213"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.270.4.F657"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116852"},{"key":"R9","unstructured":"Karakashian A, Timmer RT, Klein JD, Gunn RB, Sands JM, and Bagnasco SM.Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4.J Am Soc Nephrol10: 230\u2013237, 1999."},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Kato A, Naruse M, Knepper MA, and Sands JM.Long-term regulation of inner medullary collecting duct urea transport in rat.J Am Soc Nephrol9: 737\u2013745, 1998.","DOI":"10.1681\/ASN.V95737"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.1.F62"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00430.2003"},{"key":"R13","doi-asserted-by":"crossref","unstructured":"Kim SW, Cho SH, Oh BS, Yeum CH, Choi KC, Ahn KY, and Lee J.Diminished renal expression of aquaporin water channels in rats with experimental bilateral ureteral obstruction.J Am Soc Nephrol12: 2019\u20132028, 2001.","DOI":"10.1681\/ASN.V12102019"},{"key":"R14","unstructured":"Klahr S.Pathophysiology of obstructive nephropathy. A 1991 update.Semin Nephrol11: 156\u2013168, 1991."},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2002.00210.x"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00090.2002"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.281.1.F163"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00170.2003"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00272.2002"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Naruse M, Klein JD, Ashkar ZM, Jacobs JD, and Sands JM.Glucocorticoids downregulate the vasopressin-regulated urea transporter in rat terminal inner medullary collecting ducts.J Am Soc Nephrol8: 517\u2013523, 1997.","DOI":"10.1681\/ASN.V84517"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5495"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00331.2001"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Promeneur D, Rousselet G, Bankir L, Bailly P, Cartron JP, Ripoche P, and Trinh-Trang TM.Evidence for distinct vascular and tubular urea transporters in the rat kidney.J Am Soc Nephrol7: 852\u2013860, 1996.","DOI":"10.1681\/ASN.V76852"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-177-41912"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-197-43223"},{"key":"R26","doi-asserted-by":"crossref","unstructured":"Sands JM.Regulation of renal urea transporters.J Am Soc Nephrol10: 635\u2013646, 1999.","DOI":"10.1681\/ASN.V103635"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000035084.94743.7C"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.274.5.F978"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1997.273.3.F321"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119077"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114823"},{"key":"R32","doi-asserted-by":"crossref","unstructured":"Terris J, Ecelbarger CA, Sands JM, and Knepper MA.Long-term regulation of renal urea transporter protein expression in rat.J Am Soc Nephrol9: 729\u2013736, 1998.","DOI":"10.1681\/ASN.V95729"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.2.F325"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.4.C1318"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00359.2001"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00250.2001"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1997.17"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200207200"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1038\/365844a0"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.0054.2001"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00453.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T23:32:14Z","timestamp":1623713534000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00453.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["10.1152\/ajprenal.00453.2003"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00453.2003","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}