{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:15:24Z","timestamp":1790158524018,"version":"4.1.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)","doi-asserted-by":"publisher","award":["HL66072"],"award-info":[{"award-number":["HL66072"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)","doi-asserted-by":"publisher","award":["PO51971"],"award-info":[{"award-number":["PO51971"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"name":"American Heart Association postdoctoral fellowship","award":["11POST7450006"],"award-info":[{"award-number":["11POST7450006"]}]},{"name":"American Heart Association postdoctoral fellowship","award":["14POST18640015"],"award-info":[{"award-number":["14POST18640015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2016,7,1]]},"abstract":"<jats:p> In male rats, androgen supplements increase 20-hydroxyeicosatetraenoic acid (20-HETE) via cytochrome P-450 (CYP)4A \u03c9-hydroxylase and cause an increase in blood pressure (BP). In the present study, we determined the roles of 20-HETE and CYP4A2 on the elevated BP in hyperandrogenemic female rats. Chronic dihydrotestosterone (DHT) increased mean arterial pressure (MAP) in female Sprague-Dawley rats (96 \u00b1 2 vs. 108 \u00b1 2 mmHg, P &lt; 0.05) and was associated with increased renal microvascular CYP4A2 mRNA expression (15-fold), endogenous renal 20-HETE (5-fold), and \u03c9-hydroxylase activity (3-fold). Chronic DHT also increased MAP in low salt-fed Dahl salt-resistant female rats (81 \u00b1 4 vs. 95 \u00b1 1 mmHg, P &lt; 0.05) but had no effect on MAP in Dahl salt-sensitive female rats (154 \u00b1 3 vs. 153 \u00b1 3 mmHg), which are known to be 20-HETE deficient. To test the role of CYP4A2, female CYP4A2<jats:sup>\u2212\/\u2212<\/jats:sup> and SS.5<jats:sup>Bn<\/jats:sup> (wild type) rats were treated with DHT. DHT increased MAP in SS.5<jats:sup>Bn<\/jats:sup> female rats (104 \u00b1 1 vs. 128 \u00b1 1 mmHg, P &lt; 0.05) but had no effect in CYP4A2<jats:sup>\u2212\/\u2212<\/jats:sup> female rats (118 \u00b1 1 vs. 120 \u00b1 1 mmHg). Renal microvascular 20-HETE was reduced in control CYP4A2<jats:sup>\u2212\/\u2212<\/jats:sup> female rats and was increased with DHT in SS.5<jats:sup>Bn<\/jats:sup> female rats (6-fold) but not CYP4A2<jats:sup>\u2212\/\u2212<\/jats:sup> female rats. \u03c9-Hydroxylase activity was 40% lower in control CYP4A2<jats:sup>\u2212\/\u2212<\/jats:sup> female rats than in SS.5<jats:sup>Bn<\/jats:sup> female rats, and DHT decreased \u03c9-hydroxylase activity in SS.5<jats:sup>Bn<\/jats:sup> female rats (by 50%) but significantly increased \u03c9-hydroxylase activity in CYP4A2<jats:sup>\u2212\/\u2212<\/jats:sup> female rats (3-fold). These data suggest that 20-HETE via CYP4A2 contributes to the elevation in BP in hyperandrogenemic female rats. The data also suggest that 20-HETE synthesis inhibition may be effective in treating the elevated BP in women with hyperandrogenemia, such as women with polycystic ovary syndrome. <\/jats:p>","DOI":"10.1152\/ajprenal.00458.2015","type":"journal-article","created":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T22:39:08Z","timestamp":1463611148000},"page":"F71-F77","source":"Crossref","is-referenced-by-count":32,"title":["20-HETE and CYP4A2 \u03c9-hydroxylase contribute to the elevated blood pressure in hyperandrogenemic female rats"],"prefix":"10.1152","volume":"311","author":[{"given":"Carolina","family":"Dalmasso","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Mississippi Medical Center, Jackson, Mississippi;"},{"name":"Women's Health Research Center, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Maranon","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Mississippi Medical Center, Jackson, Mississippi;"},{"name":"Department of Nephrology, University of Mississippi Medical Center, Jackson, Mississippi;"},{"name":"Women's Health Research Center, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chetan","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Mississippi Medical Center, Jackson, Mississippi;"},{"name":"Women's Health Research Center, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohadetheh","family":"Moulana","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of Mississippi Medical Center, Jackson, Mississippi;"},{"name":"Women's Health Research Center, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian G.","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Mississippi Medical Center, Jackson, Mississippi; 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