{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:13:24Z","timestamp":1780640004973,"version":"3.54.1"},"reference-count":11,"publisher":"American Physiological Society","issue":"3","funder":[{"name":"Edmonton Civic Employees","award":["RES0046605"],"award-info":[{"award-number":["RES0046605"]}]},{"DOI":"10.13039\/100008468","name":"Canadian Urological Association","doi-asserted-by":"crossref","award":["RES0042032"],"award-info":[{"award-number":["RES0042032"]}],"id":[{"id":"10.13039\/100008468","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"<jats:p> Partial bladder outlet obstruction (pBOO) results in bladder fibrosis that is initiated by an inflammatory cascade and the decompensation after smooth muscle hypertrophy. We have been using an animal model to develop the hypothesis that mesenchymal stem cells (MSCs) are able to mitigate this cytokine cascade and prevent bladder deterioration. We hypothesized that intraperitoneal administration of MSCs can produce the same effects as intravenously administered cells but may require higher dosing. Intraperitoneal treatment will provide insights into the mechanisms of action and may offer advantages over intravenous administration, as it will permit allow higher doses and potentially reduce systemic exposure. Rats underwent a surgical induction of pBOO and instillation of either 1 \u00d7 10<jats:sup>6<\/jats:sup> or 5 \u00d7 10<jats:sup>6<\/jats:sup> commercially acquired MSCs into the peritoneum. RT-PCR, immunohistochemistry, and urodynamics were used to compare treatment groups with controls. pBOO resulted in a marked, statistically significant, upregulation of inflammatory markers in the bladder, including transforming growth factor-\u03b2, hypoxia-inducible factor-1\u03b1, hypoxia-inducible factor-3\u03b1, mammalian target of rapamycin, and collagen types I and III. Moderate but inconsistent levels of downregulation were seen with 1 \u00d7 10<jats:sup>6<\/jats:sup> MSCs, but excellent and reliable downregulation was seen with 5 \u00d7 10<jats:sup>6<\/jats:sup> MSCs ( P &lt; 0.05). Immunohistochemistry confirmed that protein levels were affected in accordance with mRNA upregulation. Urodynamics demonstrated MSC treatment resulted in whole organ physiological benefits, as they prevented elevations in detrusor pressure. In conclusion, intraperitoneal administration of MSCs resulted in a similar effect as intravenous administration; however, this required a higher dose. This has significant implications for determining the mechanism of action and potential clinical application for human therapy. <\/jats:p>","DOI":"10.1152\/ajprenal.00486.2019","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T11:54:30Z","timestamp":1578311670000},"page":"F549-F556","source":"Crossref","is-referenced-by-count":8,"title":["Intraperitoneal administration of mesenchymal stem cells is effective at mitigating detrusor deterioration after pBOO"],"prefix":"10.1152","volume":"318","author":[{"given":"Bridget","family":"Wiafe","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutuja","family":"Kadam","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7242-8178","authenticated-orcid":false,"given":"Peter D.","family":"Metcalfe","sequence":"additional","affiliation":[{"name":"Division of Urology and Pediatric Surgery, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1159\/000088310"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.5489\/cuaj.3257"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/stem.430"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.19-0044"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09445.x"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1432929100"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.02.081"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110108"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-016-0085-2"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-018-2247-1"},{"key":"B12","author":"Wiafe B","journal-title":"J Urol"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00486.2019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T07:53:16Z","timestamp":1582012396000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00486.2019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,1]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3,1]]}},"alternative-id":["10.1152\/ajprenal.00486.2019"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00486.2019","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,1]]}}}