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We have used various affinity chromatography approaches to identify the molecular targets of roscovitine and its more potent analog (S)-CR8 in human and murine polycystic kidneys. These methods revealed casein kinases 1 (CK1) as additional targets of the two drugs. CK1\u03b5 expression at the mRNA and protein levels is enhanced in polycystic kidneys of 11 different PKD mouse models as well as in human polycystic kidneys. A shift in the pattern of CK1\u03b1 isoforms is observed in all PKD mouse models. Furthermore, the catalytic activities of both CK1\u03b5 and CK1\u03b1 are increased in mouse polycystic kidneys. Inhibition of CK1\u03b5 and CK1\u03b1 may thus contribute to the long-lasting attenuating effects of roscovitine and (S)-CR8 on cyst development. CDKs and CK1s may constitute a dual therapeutic target to develop kinase inhibitory PKD drug candidates. <\/jats:p>","DOI":"10.1152\/ajprenal.00489.2017","type":"journal-article","created":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T10:30:17Z","timestamp":1521023417000},"page":"F57-F73","source":"Crossref","is-referenced-by-count":7,"title":["Casein kinase 1\u03b5 and 1\u03b1 as novel players in polycystic kidney disease and mechanistic targets for (R)-roscovitine and (S)-CR8"],"prefix":"10.1152","volume":"315","author":[{"given":"Katy","family":"Billot","sequence":"first","affiliation":[{"name":"ManRos Therapeutics, Centre de Perharidy, Roscoff, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charl\u00e8ne","family":"Coquil","sequence":"additional","affiliation":[{"name":"ManRos Therapeutics, Centre de Perharidy, Roscoff, 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