{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T22:26:27Z","timestamp":1759530387186,"version":"3.32.0"},"reference-count":51,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p>Recently, a novel Slc22 gene family member expressed in murine olfactory mucosa was identified and based on sequence homology proposed to be an organic anion transporter [Oat6 ( Slc22a20); J. C. Monte, M. A. Nagle, S. A. Eraly, and S. K. Nigam. Biochem Biophys Res Commun 323: 429\u2013436, 2004]. However, no functional data for Oat6 was reported. In the present study, we demonstrate that murine Oat6 mediates the inhibitable transport of estrone sulfate using both Xenopus oocyte expression assay and Chinese hamster ovary (CHO) cells stably transfected with mOat6 (CHO-mOat6). Uptake was virtually eliminated by probenecid and the anionic herbicide 2,4-dichlorophenoxyacetate. The organic anions ochratoxin A, salicylate, penicillin G, p-aminohippurate, and urate inhibited mOat6-mediated accumulation to varying degrees. Transport of estrone sulfate by mOat6 was demonstrated to be saturable, and K<jats:sub>m<\/jats:sub>estimates of 109.8 \u00b1 22.6 \u03bcM in oocytes and 44.8 \u00b1 7.3 \u03bcM in CHO-mOat6 cells were obtained. Inhibitory constants for 2,4-dichlorophenoxyacetate (15.7 \u00b1 2.0 \u03bcM), salicylate (49.0 \u00b1 4.4 \u03bcM), probenecid (8.3 \u00b1 2.5 \u03bcM), and penicillin G (1,450 \u00b1 480 \u03bcM) were also determined. Accumulation of estrone sulfate mediated by mOat6 was significantly trans-stimulated by glutarate, indicating that mOat6 functions as an organic anion\/dicarboxylate exchanger. These data demonstrate for the first time that the novel murine gene Oat6 ( Slc22a20) encodes a functional organic anion transporter and mOat6 is indeed the newest member of the OAT gene family.<\/jats:p>","DOI":"10.1152\/ajprenal.00497.2005","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T03:03:36Z","timestamp":1139972616000},"page":"F314-F321","source":"Crossref","is-referenced-by-count":37,"title":["Transport of estrone sulfate by the novel organic anion transporter Oat6 (<i>Slc22a20<\/i>)"],"prefix":"10.1152","volume":"291","author":[{"given":"Gloriane W.","family":"Schnabolk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geri L.","family":"Youngblood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas H.","family":"Sweet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.088583"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Apiwattanakul N, Sekine T, Chairoungdua A, Kanai Y, Nakajima N, Sophasan S, and Endou H.Transport properties of nonsteroidal anti-inflammatory drugs by organic anion transporter 1 expressed in Xenopus laevis oocytes.Mol Pharmacol55: 847\u2013854, 1999.","DOI":"10.1016\/S0026-895X(24)23181-3"},{"key":"R4","unstructured":"Carola R, Harley JP, and Noback CR.Human Anatomy and Physiology. New York: McGraw Hill, 1992."},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1124\/mol.59.5.1277"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.6.4507"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(98)00170-7"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1002\/jps.1122"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1021\/js9900295"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1124\/mol.56.3.570"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007542618378"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(98)00095-8"},{"key":"R13","unstructured":"Dudley AW Jrand Thapar NT.Fatal human ingestion of 2,4-D, a common herbicide.Arch Pathol94: 270\u2013275, 1972."},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(79)90368-5"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.301.3.797"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02853-X"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.3109\/10611869909085497"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00117.2003"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-004-9013-3"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025053115583"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.300.3.746"},{"key":"R22","doi-asserted-by":"crossref","unstructured":"Hashimoto T, Narikawa S, Huang XL, Minematsu T, Usui T, Kamimura H, and Endou H.Characterization of the renal tubular transport of zonampanel, a novel alpha-amino-3hydroxy-5-methylisoxazole-4-propionic acid receptor antagonist, by human organic anion transporters.Drug Metab Dispos32: 1096\u20131102, 2004.","DOI":"10.1124\/dmd.32.10.1096"},{"key":"R23","unstructured":"Jariyawat S, Sekine T, Takeda M, Apiwattanakul N, Kanai Y, Sophasan S, and Endou H.The interaction and transport of beta-lactam antibiotics with the cloned rat renal organic anion transporter 1.J Pharmacol Exp Ther290: 672\u2013677, 1999."},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20284"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-005-5420-3"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1124\/mol.62.1.7"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.1.C289"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.19.13675"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2005.06.028"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.033175"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.08.112"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000050062.57184.75"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20175"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.47.33382"},{"key":"R35","unstructured":"Sambrook Jand Russell DW.Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 2001."},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00585-7"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.30.18526"},{"key":"R38","doi-asserted-by":"crossref","unstructured":"Simonson GD, Vincent AC, Roberg KJ, Huang Y, and Iwanij V.Molecular cloning and characterization of a novel liver-specific transport protein.J Cell Sci107: 1065\u20131072, 1994.","DOI":"10.1242\/jcs.107.4.1065"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.10.016"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.281.2.F197"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00405.2002"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.6.F864"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203803200"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1007\/BF02738157"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.277.6.F890"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.48.30088"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(01)00962-1"},{"key":"R48","doi-asserted-by":"crossref","unstructured":"Tsuda M, Sekine T, Takeda M, Cha SH, Kanai Y, Kimura M, and Endou H.Transport of ochratoxin A by renal multispecific organic anion transporter 1.J Pharmacol Exp Ther289: 1301\u20131305, 1999.","DOI":"10.1016\/S0022-3565(24)38273-4"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(03)00341-7"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00040.2003"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00396.2003"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00497.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T19:21:06Z","timestamp":1736277666000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00497.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1152\/ajprenal.00497.2005"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00497.2005","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"type":"print","value":"1931-857X"},{"type":"electronic","value":"1522-1466"}],"subject":[],"published":{"date-parts":[[2006,8]]}}}