{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:29:02Z","timestamp":1778275742779,"version":"3.51.4"},"reference-count":46,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,2,1]]},"abstract":"<jats:p> Tubuloglomerular feedback (TGF)-mediated constriction of the afferent arteriole is modulated by a balance between release of superoxide (O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>) and nitric oxide (NO) in macula densa (MD) cells. Aldosterone activates mineralocorticoid receptors that are expressed in the MD and induces both NO and O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> generation. We hypothesize that aldosterone enhances O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production in the MD mediated by protein kinase C (PKC), which buffers the effect of NO in control of TGF response. Studies were performed in microdissected and perfused MD and in a MD cell line, MMDD1 cells. Aldosterone significantly enhanced O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> generation both in perfused MD and in MMDD1 cells. When aldosterone (10<jats:sup>\u22127<\/jats:sup> mol\/l) was added in the tubular perfusate, TGF response was reduced from 2.4 \u00b1 0.3 \u03bcm to 1.4 \u00b1 0.2 \u03bcm in isolated perfused MD. In the presence of tempol, a O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> scavenger, TGF response was 1.5 \u00b1 0.2 \u03bcm. In the presence of both tempol and aldosterone in the tubular perfusate, TGF response was further reduced to 0.4 \u00b1 0.2 \u03bcm. To determine if PKC is involved in aldosterone-induced O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production, we exposed the O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> cells to a nonselective PKC inhibitor chelerythrine chloride, a specific PKC\u03b1 inhibitor Go6976, or a PKC\u03b1 siRNA, and the aldosterone-induced increase in O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production was blocked. These data indicate that aldosterone-stimulated O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> production in the MD buffers the effect of NO in control of TGF response, an effect that was mediated by PKC\u03b1. <\/jats:p>","DOI":"10.1152\/ajprenal.00501.2012","type":"journal-article","created":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T05:50:05Z","timestamp":1354773005000},"page":"F326-F332","source":"Crossref","is-referenced-by-count":16,"title":["Interaction between nitric oxide and superoxide in the macula densa in aldosterone-induced alterations of tubuloglomerular feedback"],"prefix":"10.1152","volume":"304","author":[{"given":"Qian","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"},{"name":"Department of Cardiac Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhua","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengwei","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; and"},{"name":"Department of Cardiac Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"R. 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