{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:14:48Z","timestamp":1758820488511,"version":"3.40.4"},"reference-count":38,"publisher":"American Physiological Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:p>The purpose of this study was to determine whether duloxetine [a serotonin (5-HT)-norepinephrine reuptake inhibitor] combined with transcutaneous foot stimulation or WAY-100635 (a 5-HT<jats:sub>1A<\/jats:sub>antagonist) can enhance inhibition of bladder overactivity in cats. Cystometrograms were performed on eight cats under \u03b1-chloralose anesthesia by infusing saline and then 0.25% acetic acid (AA) to induce bladder overactivity. To inhibit bladder overactivity, foot stimulation (5 Hz) was applied via transcutaneous pad electrodes to the right hindfoot at two and four times the threshold intensity for inducing a toe twitch. Duloxetine (0.003\u20133 mg\/kg) was administered intravenously to determine the effect of combination treatment. After the 3 mg\/kg dose of duloxetine, WAY-100635 (0.5 mg\/kg) was given intravenously. AA irritation significantly ( P &lt; 0.0001) reduced bladder capacity to 42.7 \u00b1 7.4% of the saline control capacity. Foot stimulation alone at both two and four times the threshold intensity significantly ( P &lt; 0.0001) inhibited bladder overactivity and increased bladder capacity to 66.7 \u00b1 6.3% and 85.7 \u00b1 6.5% of the saline control, respectively. Duloxetine alone dose dependently inhibited bladder overactivity and completely restored bladder capacity to the saline control (109 \u00b1 15.5%) at 3 mg\/kg. Although duloxetine combined with foot stimulation did not further enhance inhibition, WAY-100635 (0.5 mg\/kg) given after 3 mg\/kg duloxetine further increased ( P = 0.008) bladder capacity to 162.2 \u00b1 22.5% of the saline control. Although duloxetine and foot stimulation independently inhibited bladder overactivity, combined treatment did not enhance inhibition. Duloxetine combined with WAY-100635, however, synergistically enhanced bladder inhibition, indicating a potential novel treatment for overactive bladder if duloxetine is combined with a 5-HT<jats:sub>1A<\/jats:sub>receptor antagonist drug.<\/jats:p>","DOI":"10.1152\/ajprenal.00523.2013","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T02:56:45Z","timestamp":1382583405000},"page":"F1663-F1668","source":"Crossref","is-referenced-by-count":8,"title":["Inhibition of bladder overactivity by duloxetine in combination with foot stimulation or WAY-100635 treatment in cats"],"prefix":"10.1152","volume":"305","author":[{"given":"Zeyad","family":"Schwen","sequence":"first","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosuke","family":"Matsuta","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jicheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James R.","family":"Roppolo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"de Groat","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changfeng","family":"Tai","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; and"},{"name":"Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2003.10.005"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00622-1"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2005.01.019"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1533-2500.2010.00401.x"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.06.047"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.09.012"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1993.265.4.F520"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1097\/WNF.0b013e3182613dce"},{"key":"B9","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/S0022-3565(25)20660-7","volume":"278","author":"Fornal CA","year":"1996","journal-title":"J Pharmacol Exp Ther"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2401"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb15919.x"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1007\/s002130050238"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1097\/SPV.0b013e31820e5cf3"},{"key":"B14","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1016\/S0022-3565(25)09547-3","volume":"273","author":"Gobert A","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200443130-00004"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.070656"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.075960"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.07.110"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2004.02.041"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.05.006"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90865-4"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410x.2001.02228.x"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01334.x"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.124840"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.05.045"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2008.02.024"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22479"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00105.2013"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.06980.x"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)63300-7"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.01.007"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.04.096"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09358.x"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(03)00754-4"},{"key":"B35","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/S0022-3565(25)10548-X","volume":"274","author":"Thor KB","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)02897-4"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.07.032"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000240225.04000.1a"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.00523.2013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T18:10:02Z","timestamp":1746036602000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.00523.2013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,15]]},"references-count":38,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12,15]]}},"alternative-id":["10.1152\/ajprenal.00523.2013"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.00523.2013","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"type":"print","value":"1931-857X"},{"type":"electronic","value":"1522-1466"}],"subject":[],"published":{"date-parts":[[2013,12,15]]}}}