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This model is characterized by acute-phase inflammation (neutrophil and macrophage infiltration on days 0\u20133) and late-phase PF (\u03b1-smooth muscle actin-positive fibroblast infiltration, type III collagen accumulation, and neoangiogenesis on days 7\u201314). Peritoneal thickening peaked on day 14. MR was expressed in rat peritoneum and a rat fibroblast cell line. Expression of its effector kinase [serum- and glucocorticoid-induced kinase-1 (Sgk1)], transforming growth factor-\u03b2 (TGF-\u03b2), plasminogen activator inhibitor-1 (PAI-1), and CD31-positive vessels increased during the course of PF. Rats were treated with spironolactone, angiotensin receptor blockade (ARB), or angiotensin-converting enzyme inhibitor (ACEI)-ARB-spironolactone starting at 6 h after peritoneal scraping. All parameters, including peritoneal thickening, number of macrophages and CD31-positive vessels, and expression of monocyte chemoattractant protein-1, TGF-\u03b2, PAI-1, and Sgk1, were significantly suppressed by spironolactone (10 mg\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup>). The effects of spironolactone (10 and 20 mg\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup>) were very similar to those of triple blockade. ARB, but not ACEI, significantly reduced peritoneal thickening. Furthermore, peritoneal function assessed by peritoneal equilibration test was significantly improved by spironolactone. Our results suggest that MR is a potential target to prevent inflammation-induced PF in patients on peritoneal dialysis.<\/jats:p>","DOI":"10.1152\/ajprenal.00565.2007","type":"journal-article","created":{"date-parts":[[2008,3,20]],"date-time":"2008-03-20T00:46:31Z","timestamp":1205973991000},"page":"F1084-F1093","source":"Crossref","is-referenced-by-count":43,"title":["Mineralocorticoid receptor blockade ameliorates peritoneal fibrosis in new rat peritonitis 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