{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T22:43:04Z","timestamp":1781649784597,"version":"3.54.5"},"reference-count":26,"publisher":"American Physiological Society","issue":"6","funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81260120"],"award-info":[{"award-number":["81260120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81500586"],"award-info":[{"award-number":["81500586"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2018,6,1]]},"abstract":"<jats:p> Although epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells was recognized as the key process of peritoneal fibrosis, which is a major cause of peritoneal failure related to peritoneal dialysis (PD), mechanisms underlying these processes remain largely unknown. In this study, we found that miR-200a was significantly downregulated in peritoneal tissues with fibrosis in a rat model of PD. In vitro, transforming growth factor (TGF)-\u03b2<jats:sub>1<\/jats:sub>-induced EMT, identified by de novo expression of \u03b1-smooth muscle actin and a loss of E-cadherin in human peritoneal mesothelial cells (HPMCs), was associated with downregulation of miR-200a but upregulation of zinc finger E-box-binding homeobox 1\/2 (ZEB1\/2), suggesting a close link between miR-200a and ZEB1\/2 in TGF-\u03b2<jats:sub>1<\/jats:sub>-induced EMT. It was further demonstrated that miR-200a was able to bind to the 3\u2032UTR of ZEB1\/2, and overexpression of miR-200a blocked TGF-\u03b2<jats:sub>1<\/jats:sub>-induced upregulation of ZEB1\/2 and, therefore, inhibited EMT and collagen expression. In contrast, overexpression ZEB1\/2 blocked miR-200a inhibition of EMT and collagen expression in HMPCs. In conclusion, miR-200a could negatively regulate TGF-\u03b2<jats:sub>1<\/jats:sub>-induced EMT by targeting ZEB1\/2 in peritoneal mesothelial cells. Blockade of EMT in HPMCS indicates the therapeutic potential of miR-200a as a treatment for peritoneal fibrosis associated with PD. <\/jats:p>","DOI":"10.1152\/ajprenal.00566.2016","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T08:59:07Z","timestamp":1515574747000},"page":"F1087-F1095","source":"Crossref","is-referenced-by-count":20,"title":["MiR-200a negatively regulates TGF-\u03b2<sub>1<\/sub>-induced epithelial-mesenchymal transition of peritoneal mesothelial cells by targeting ZEB1\/2 expression"],"prefix":"10.1152","volume":"314","author":[{"given":"Runsheng","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojun","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The First Affiliated Hospital of Nanchang University, Nanchang, 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