{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T20:46:31Z","timestamp":1778273191936,"version":"3.51.4"},"reference-count":42,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:p>Amiloride-sensitive Na<jats:sup>+<\/jats:sup>channel activity was examined in the cortical collecting ducts of a mouse line (SGK1<jats:sup>\u2212\/\u2212<\/jats:sup>) deficient in the serum- and glucocorticoid-dependent protein kinase SGK1. This activity was correlated with changes in renal Na handling and in the maturation of epithelial Na<jats:sup>+<\/jats:sup>channel (ENaC) protein. Neither SGK1<jats:sup>\u2212\/\u2212<\/jats:sup>mice nor paired SGK1<jats:sup>+\/+<\/jats:sup>animals expressed detectable channel activity, measured as amiloride-sensitive whole-cell current ( I<jats:sub>Na<\/jats:sub>), under control conditions with standard chow. Administration of aldosterone (0.5 \u03bcg\/h via osmotic minipump for 7 days) increased I<jats:sub>Na<\/jats:sub>to a similar extent in SGK1<jats:sup>+\/+<\/jats:sup>(378 \u00b1 61 pA\/cell at \u2212100 mV) and in SGK1<jats:sup>\u2212\/\u2212<\/jats:sup>(350 \u00b1 57 pA\/cell) animals. However, the maturation of ENaC, assessed as the ratio of cleaved to full-length forms of \u03b3-ENaC, was more pronounced in SGK<jats:sup>+\/+<\/jats:sup>mice. The SGK1<jats:sup>\u2212\/\u2212<\/jats:sup>animals exhibited a salt-wasting phenotype when kept on a low-Na diet for up to 2 days, losing significantly more Na in the urine than wild-type mice. Under these conditions, I<jats:sub>Na<\/jats:sub>was enhanced more in SGK1<jats:sup>\u2212\/\u2212<\/jats:sup>(94 \u00b1 14 pA\/cell) than in SGK<jats:sup>+\/+<\/jats:sup>(23 \u00b1 5 pA\/cell) genotypes. Despite the larger currents, the ratio of cleaved to full-length \u03b3-ENaC was lower in the knockout animals. The mice also expressed a smaller amount of Na<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>cotransporter protein under Na-depleted conditions. 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