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Intravesical administration of 40 and 130 \u03bcM \u03b1,\u03b2-meATP increased afferent firing from 27 \u00b1 3 to 53 \u00b1 6 and 61 \u00b1 2 spikes\/s, respectively, but did not change the maximum amplitude of spontaneous bladder contractions. Electrical stimulation on the surface of the bladder elicited action potentials (AP) in BAN. \u03b1,\u03b2-meATP decreased the voltage threshold from 9.0 \u00b1 1.2 to 3.5 \u00b1 0.5 V (0.15-ms pulse duration) and increased the area of the APs (82% at 80-V stimulus intensity). These effects were blocked by TNP-ATP (30 \u03bcM). ATP (2 mM) applied in the bath produced similar changes in BAN activity. These effects were blocked by bath application of PPADS (30 \u03bcM). Neither TNP-ATP nor PPADS affected BAN activity induced by distension of the bladder. Cystitis induced by pretreatment of the rats with cyclophosphamide (100 mg\/kg ip) increased afferent firing in response to isotonic bladder distension (10\u201340 cmH<jats:sub>2<\/jats:sub>O), decreased the threshold, and increased the area of evoked APs. The increase in afferent firing at 10 cmH<jats:sub>2<\/jats:sub>O intravesical pressure was reduced 52% by PPADS. These results indicate that purinergic agonists acting on P2X receptors and cystitis induced by cyclophosphamide can increase excitability of the BANs.<\/jats:p>","DOI":"10.1152\/ajprenal.00592.2007","type":"journal-article","created":{"date-parts":[[2008,3,6]],"date-time":"2008-03-06T01:59:01Z","timestamp":1204768741000},"page":"F1146-F1156","source":"Crossref","is-referenced-by-count":71,"title":["Sensitization of pelvic afferent nerves in the in vitro rat urinary bladder-pelvic nerve preparation by purinergic agonists and cyclophosphamide pretreatment"],"prefix":"10.1152","volume":"294","author":[{"given":"Yongbei","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"de Groat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb14845.x"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00056.2003"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00118.2004"},{"key":"R4","doi-asserted-by":"crossref","unstructured":"Borlak J, Zwadlo C.The myosin ATPase inhibitor 2,3-Butanedione monoxime dictates transcriptional activation of ion channels and Ca2+-handling proteins.Mol Pharmacol66: 708\u2013717, 2004.","DOI":"10.1016\/S0026-895X(24)05655-4"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)02003-X"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bja.a013473"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91082-X"},{"key":"R8","unstructured":"Burnstock G.Purinergic signalling in lower urinary tract. 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