{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:59:23Z","timestamp":1740139163530,"version":"3.37.3"},"reference-count":27,"publisher":"American Physiological Society","issue":"8","funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)","doi-asserted-by":"publisher","award":["DK-094905","DK-090006","DK-102427","DK-091253"],"award-info":[{"award-number":["DK-094905","DK-090006","DK-102427","DK-091253"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2015,4,15]]},"abstract":"<jats:p> This study examined the role of spinal metabotropic glutamate receptor 5 (mGluR5) in the nociceptive C-fiber afferent-mediated spinal bladder reflex and in the inhibtion of this reflex by pudendal nerve stimulation (PNS). In \u03b1-chloralose-anesthetized cats after spinal cord transection at the T9\/T10 level, intravesical infusion of 0.25% acetic acid irritated the bladder, activated nociceptive C-fiber afferents, and induced spinal reflex bladder contractions of low amplitude (&lt;50 cmH<jats:sub>2<\/jats:sub>O) and short duration (&lt;20 s) at a smaller bladder capacity \u223c80% of saline control capacity. PNS significantly ( P &lt; 0.01) increased bladder capacity from 85.5 \u00b1 10.1 to 137.3 \u00b1 14.1 or 148.2 \u00b1 11.2% at 2T or 4T stimulation, respectively, where T is the threshold intensity for PNS to induce anal twitch. MTEP {3-[(2-methyl-4-thiazolyl)ethynyl]pyridine; 3 mg\/kg iv, a selective mGluR5 antagonist} completely removed the PNS inhibition and significantly ( P &lt; 0.05) increased bladder capacity from 71.8 \u00b1 9.9 to 94.0 \u00b1 13.9% of saline control, but it did not change the bladder contraction amplitude. After propranolol (3 mg\/kg iv, a \u03b21\/\u03b22-adrenergic receptor antagonist) treatment, PNS inhibition remained but MTEP significantly ( P &lt; 0.05) reduced the bladder contraction amplitude from 18.6 \u00b1 2.1 to 6.6 \u00b1 1.2 cmH<jats:sub>2<\/jats:sub>O and eliminated PNS inhibition. At the end of experiments, hexamethonium (10 mg\/kg iv, a ganglionic blocker) significantly ( P &lt; 0.05) reduced the bladder contraction amplitude from 20.9 \u00b1 3.2 to 8.1 \u00b1 1.5 cmH<jats:sub>2<\/jats:sub>O on average demonstrating that spinal reflexes were responsible for a major component of the contractions. This study shows that spinal mGluR5 plays an important role in the nociceptive C-fiber afferent-mediated spinal bladder reflex and in pudendal inhibition of this spinal reflex. <\/jats:p>","DOI":"10.1152\/ajprenal.00623.2014","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T00:38:04Z","timestamp":1423701484000},"page":"F832-F838","source":"Crossref","is-referenced-by-count":7,"title":["Role of spinal metabotropic glutamate receptor 5 in pudendal inhibition of the nociceptive bladder reflex in cats"],"prefix":"10.1152","volume":"308","author":[{"given":"Jeremy N.","family":"Reese","sequence":"first","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc J.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiying","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania;"},{"name":"Department of Urology, The Second Hospital, Shandong University, Jinan, People's Republic of China; 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