{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:27:11Z","timestamp":1790054831208,"version":"4.0.1"},"reference-count":41,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2014,7,15]]},"abstract":"<jats:p> An increase in tubular fluid flow rate (TFF) stimulates Na reabsorption and K secretion in the cortical collecting duct (CCD) and subjects cells therein to biomechanical forces including fluid shear stress (FSS) and circumferential stretch (CS). Intracellular MAPK and extracellular autocrine\/paracrine PGE<jats:sub>2<\/jats:sub> signaling regulate cation transport in the CCD and, at least in other systems, are affected by biomechanical forces. We hypothesized that FSS and CS differentially affect MAPK signaling and PGE<jats:sub>2<\/jats:sub> release to modulate cation transport in the CCD. To validate that CS is a physiological force in vivo, we applied the intravital microscopic approach to rodent kidneys in vivo to show that saline or furosemide injection led to a 46.5 \u00b1 2.0 or 170 \u00b1 32% increase, respectively, in distal tubular diameter. Next, murine CCD (mpkCCD) cells were grown on glass or silicone coated with collagen type IV and subjected to 0 or 0.4 dyne\/cm<jats:sup>2<\/jats:sup> of FSS or 10% CS, respectively, forces chosen based on prior biomechanical modeling of ex vivo microperfused CCDs. Cells exposed to FSS expressed an approximately twofold greater abundance of phospho(p)-ERK and p-p38 vs. static cells, while CS did not alter p-p38 and p-ERK expression compared with unstretched controls. FSS induced whereas CS reduced PGE<jats:sub>2<\/jats:sub> release by \u223c40%. In conclusion, FSS and CS differentially affect ERK and p38 activation and PGE<jats:sub>2<\/jats:sub> release in a cell culture model of the CD. We speculate that TFF differentially regulates biomechanical signaling and, in turn, cation transport in the CCD. <\/jats:p>","DOI":"10.1152\/ajprenal.00634.2013","type":"journal-article","created":{"date-parts":[[2014,5,29]],"date-time":"2014-05-29T07:40:18Z","timestamp":1401349218000},"page":"F195-F204","source":"Crossref","is-referenced-by-count":35,"title":["Effects of biomechanical forces on signaling in the cortical collecting duct (CCD)"],"prefix":"10.1152","volume":"307","author":[{"given":"Rolando","family":"Carrisoza-Gaytan","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Flores","sequence":"additional","affiliation":[{"name":"Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York;"},{"name":"Department of Medicine, James J. 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