{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:15:00Z","timestamp":1790957700191,"version":"4.1.0"},"reference-count":43,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>An inorganic phosphate (P<jats:sub>i<\/jats:sub>)-restricted diet is important for patients with chronic kidney disease and patients on hemodialysis. Phosphate binders are essential for preventing hyperphosphatemia and ectopic calcification. The sodium-dependent P<jats:sub>i<\/jats:sub>(Na\/P<jats:sub>i<\/jats:sub>) transport system is involved in intestinal P<jats:sub>i<\/jats:sub>absorption and is regulated by several factors. The type II sodium-dependent P<jats:sub>i<\/jats:sub>transporter Npt2b is expressed in the brush-border membrane in intestinal epithelial cells and transports P<jats:sub>i<\/jats:sub>. In the present study, we analyzed the phenotype of Npt2b<jats:sup>\u2212\/\u2212<\/jats:sup>and hetero<jats:sup>+\/\u2212<\/jats:sup>mice. Npt2b<jats:sup>\u2212\/\u2212<\/jats:sup>mice died in utero soon after implantation, indicating that Npt2b is essential for early embryonic development. At 4 wk of age, Npt2b<jats:sup>+\/\u2212<\/jats:sup>mice showed hypophosphatemia and low urinary P<jats:sub>i<\/jats:sub>excretion. Plasma fibroblast growth factor 23 levels were significantly decreased and 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>levels were significantly increased in Npt2b<jats:sup>+\/\u2212<\/jats:sup>mice compared with Npt2b<jats:sup>+\/+<\/jats:sup>mice. Npt2b mRNA levels were reduced to 50% that in Npt2b<jats:sup>+\/+<\/jats:sup>mice. In contrast, renal Npt2a and Npt2c transporter protein levels were significantly increased in Npt2b<jats:sup>+\/\u2212<\/jats:sup>mice. At 20 wk of age, Npt2b<jats:sup>+\/\u2212<\/jats:sup>mice showed hypophosphaturia and reduced Na\/P<jats:sub>i<\/jats:sub>cotransport activity in the distal intestine. Npt2b<jats:sup>+\/+<\/jats:sup>mice with adenine-induced renal failure had hyperphosphatemia and high plasma creatinine levels. Npt2b<jats:sup>+\/\u2212<\/jats:sup>mice treated with adenine had significantly reduced plasma P<jats:sub>i<\/jats:sub>levels compared with Npt2b<jats:sup>+\/+<\/jats:sup>mice. Intestinal Npt2b protein and Na<jats:sup>+<\/jats:sup>\/P<jats:sub>i<\/jats:sub>transport activity levels were significantly lower in Npt2b<jats:sup>+\/\u2212<\/jats:sup>mice than in the Npt2b<jats:sup>+\/+<\/jats:sup>mice. The findings of the present studies suggest that Npt2b is an important target for the prevention of hyperphosphatemia.<\/jats:p>","DOI":"10.1152\/ajprenal.00663.2010","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T04:24:58Z","timestamp":1312431898000},"page":"F1105-F1113","source":"Crossref","is-referenced-by-count":41,"title":["Inorganic phosphate homeostasis in sodium-dependent phosphate cotransporter Npt2b<sup>+\/\u2212<\/sup>mice"],"prefix":"10.1152","volume":"301","author":[{"given":"Akiko","family":"Ohi","sequence":"first","affiliation":[{"name":"Department of Molecular Nutrition Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima City; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etsuyo","family":"Hanabusa","sequence":"additional","affiliation":[{"name":"Department of Molecular Nutrition Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima City; 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