{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:22:44Z","timestamp":1778275364042,"version":"3.51.4"},"reference-count":36,"publisher":"American Physiological Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2012,5,15]]},"abstract":"<jats:p>We tested the effects of insulin (2 nM, 30\u201360 min) on principal cells of isolated split-open rat cortical collecting ducts (CCD) using whole-cell current measurements. Insulin addition to the superfusate of the tubules enhanced Na pump (ouabain-sensitive) current from 18 \u00b1 3 to 31 \u00b1 3 pA\/cell in control and from 74 \u00b1 9 to 126 \u00b1 11 pA\/cell in high K-fed animals. It also more than doubled ROMK (tertiapin-Q-sensitive) K<jats:sup>+<\/jats:sup>currents in control CCD from 320 \u00b1 40 to 700 \u00b1 80 pA\/cell, although it did not affect this current in tubules from K-loaded rats. Insulin did not induce the appearance of amiloride-sensitive Na<jats:sup>+<\/jats:sup>current in control animals, while in high K-fed animals the currents were similar in the presence (140 \u00b1 30) and the absence (180 \u00b1 70 pA\/cell) of insulin. Intraperitoneal injection of insulin plus hypertonic dextrose decreased Na excretion, as previously reported. However, injection of dextrose alone, or the nonmetabolized sugar mannose, had similar effects, suggesting that they were largely the result of vascular volume depletion rather than specific actions of the hormone. In summary, we find no evidence for acute upregulation of the epithelial Na channel (ENaC) by physiological concentrations of insulin in the mammalian CCD. However, the hormone does activate both the Na\/K pump and apical K<jats:sup>+<\/jats:sup>channels and could, under some conditions, enhance renal K<jats:sup>+<\/jats:sup>secretion.<\/jats:p>","DOI":"10.1152\/ajprenal.00675.2011","type":"journal-article","created":{"date-parts":[[2012,2,23]],"date-time":"2012-02-23T07:02:32Z","timestamp":1329980552000},"page":"F1227-F1233","source":"Crossref","is-referenced-by-count":37,"title":["Effects of insulin on Na and K transporters in the rat CCD"],"prefix":"10.1152","volume":"302","author":[{"given":"Gustavo","family":"Frindt","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence G.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New 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