{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T06:57:20Z","timestamp":1758265040384},"reference-count":38,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:p> Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the curent study was to analyze effects of BMP-5 treatment in an eEOC-based therapy of murine AKI and 5\/6-nephrectomy. Male C57\/Bl6N mice were either subjected to unilateral renal artery clamping postuninephrectomy or to 5\/6-nephrectomy. Untreated or BMP-5-pretreated murine eEOCs were injected into recipient animals at the time of reperfusion (AKI) or at 2 and 5 days after 5\/6-nephrectomy. Analysis of renal function and morphology was performed at 48 h and at 6 wk (AKI) or at 8 wk (5\/6 model). Cellular consequences of eEOC treatment were evaluated using different in vitro assays. AKI was mitigated significantly by injecting BMP-5-pretreated eEOCs. Renal function was improved at 48 h and at 6 wk after cell therapy. In 5\/6-nephrectomy, the cells failed to protect renal function, but proteinuria was reduced after administering untreated eEOCs. BMP-5 pretreatment resulted in aggravated proteinuria and renal fibrosis. In 5\/6-nephrectomized animals, percentages of anti-smooth muscle actin+\/CD31+ cells increased, indicating endothelial-mesenchymal transition (EnMT). In vitro analysis revealed increased cell migration and reduced cell apoptosis\/necrosis. Paracrinic activity remained unaffected. BMP-5 acts as a potent eEOC agonist in murine AKI in the short and mid to long term. Cell effects in 5\/6-nephrectomy are heterogenous, but untreated cells act antiproteinurically and antifibrotically without any impact on EnMT. <\/jats:p>","DOI":"10.1152\/ajprenal.00677.2012","type":"journal-article","created":{"date-parts":[[2013,5,16]],"date-time":"2013-05-16T04:22:37Z","timestamp":1368678157000},"page":"F314-F322","source":"Crossref","is-referenced-by-count":17,"title":["Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5\/6-chronic kidney disease"],"prefix":"10.1152","volume":"305","author":[{"given":"Daniel","family":"Patschan","sequence":"first","affiliation":[{"name":"Department of Nephrology and Rheumatology, University Hospital of G\u00f6ttingen, G\u00f6ttingen, Germany; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Schwarze","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Rheumatology, University Hospital of G\u00f6ttingen, G\u00f6ttingen, Germany; 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