{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:22:45Z","timestamp":1778275365563,"version":"3.51.4"},"reference-count":85,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>The fine regulation of Na<jats:sup>+<\/jats:sup>and K<jats:sup>+<\/jats:sup>transport takes place in the cortical distal nephron. It is well established that K<jats:sup>+<\/jats:sup>secretion occurs through apical K<jats:sup>+<\/jats:sup>channels: the ROMK and the Ca<jats:sup>2+<\/jats:sup>- and voltage-dependent maxi-K. Previously, we identified the voltage-gated Kv1.3 channel in the inner medulla of the rat kidney (Escobar LI, Mart\u00ednez-T\u00e9llez JC, Salas M, Castilla SA, Carrisoza R, Tapia D, V\u00e1zquez M, Bargas J, Bol\u00edvar JJ. Am J Physiol Cell Physiol 286: C965\u2013C974, 2004). To examine the role of Kv1.3 in the renal regulation of K<jats:sup>+<\/jats:sup>homeostasis, we characterized the effect of dietary K<jats:sup>+<\/jats:sup>on the molecular and functional expression of this channel. We performed real-time-PCR and immunoblot assays in kidneys from rats fed a control (CK; 1.2% wt\/wt) or high-K<jats:sup>+<\/jats:sup>(HK; 10% wt\/wt) diet for 5\u201315 days. Kv1.3 mRNA and protein expression did not change with HK in the whole kidney. However, dietary K<jats:sup>+<\/jats:sup>loading provoked a change in the cellular distribution of Kv1.3 from the cytoplasm to apical membranes. Immunolocalization of Kv1.3 detected the channel exclusively in the intercalated cells. We investigated whether Kv1.3 mediated K<jats:sup>+<\/jats:sup>transport in microperfused cortical collecting ducts (CCDs). The HK diet led to an increase in net K<jats:sup>+<\/jats:sup>transport from 7.4 \u00b1 1.1 (CK) to 11.4 \u00b1 1.0 (HK) pmol\u00b7min<jats:sup>\u22121\u00b7<\/jats:sup>mm<jats:sup>\u22121<\/jats:sup>. Luminal margatoxin, a specific blocker of Kv1.3, decreased net K<jats:sup>+<\/jats:sup>secretion in HK CCDs to 6.0 \u00b1 1.6 pmol\u00b7min<jats:sup>\u22121\u00b7<\/jats:sup>mm<jats:sup>\u22121<\/jats:sup>. Our data provide the first evidence that Kv1.3 channels participate in K<jats:sup>+<\/jats:sup>secretion and that apical membrane localization of Kv1.3 is enhanced in the intercalated cells by dietary K<jats:sup>+<\/jats:sup>loading.<\/jats:p>","DOI":"10.1152\/ajprenal.00697.2009","type":"journal-article","created":{"date-parts":[[2010,4,29]],"date-time":"2010-04-29T02:14:22Z","timestamp":1272507262000},"page":"F255-F264","source":"Crossref","is-referenced-by-count":21,"title":["Potassium secretion by voltage-gated potassium channel Kv1.3 in the rat kidney"],"prefix":"10.1152","volume":"299","author":[{"given":"Rolando","family":"Carrisoza-Gayt\u00e1n","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Salvador","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, 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