{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T09:16:55Z","timestamp":1649063815773},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[1981,4,1]]},"abstract":"<jats:p> We measured titratable acid (TA) and NH4 excretion by isolated rat kidneys perfused either with conventional bicarbonate-containing solutions or with solutions in which bicarbonate was replaced by propionate. Rates of TA excretion by bicarbonate-perfused kidneys were similar to in vivo values, 0.27 +\/- 0.04 mueq.ml GF-1 (0.21 mueq.min-1.g-1), and increased significantly under bicarbonate-free conditions to 0.70 +\/- 0.12 mueq.ml GF-1 (0.42 mueq.min-1.g-1). At the same time the perfusate\/urine pH difference (delta pH) increased significantly, from 0.63 +\/- 0.06 to 0.92 +\/- 0.06. Carbonic anhydrase inhibition by 5 X 10(-4) M acetazolamide alkalinized the urine of bicarbonate-perfused kidneys, while in the bicarbonate-free preparation the urine remained acid (delta pH = 0.27 +\/- 0.04) and titratable acid continued to be excreted, though at a reduced rate, 0.19 +\/- 0.04 mueq.ml GF-1. Under these same bicarbonate-free carbonic anhydrase-inhibited conditions, lowering the perfusate pH from 7.4 to 7.1 increased delta pH to 0.36 +\/- 0.02 and caused total acid excretion (TA + NH4) to rise from 0.29 +\/- 0.04 to 0.45 +\/- 0.06 mueq.ml GF-1, and increasing the perfusate [HPO4] from 2.4 to 9.6 mM increased TA to 0.80 +\/- 0.09 mueq.ml GF-1. <\/jats:p>","DOI":"10.1152\/ajprenal.1981.240.4.f306","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T12:45:10Z","timestamp":1513946710000},"page":"F306-F310","source":"Crossref","is-referenced-by-count":0,"title":["Acid excretion by bicarbonate-free perfused rat kidney"],"prefix":"10.1152","volume":"240","author":[{"given":"M. H.","family":"Garvey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Maude","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.1981.240.4.F306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:09:31Z","timestamp":1567966171000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.1981.240.4.F306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1981,4,1]]}},"alternative-id":["10.1152\/ajprenal.1981.240.4.F306"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.1981.240.4.f306","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,4,1]]}}}