{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T14:08:18Z","timestamp":1648994898407},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[1985,2,1]]},"abstract":"<jats:p> Extracts of mammalian atrial tissue contain potent natriuretic substances known collectively as atrial natriuretic factor (ANF). The purposes of the present experiments were: 1) to improve on existing bioassay methodology for the detection of ANF activity in atrial extracts, and 2) to compare the ANF activity of atrial extracts prepared from Brattleboro-stain diabetes insipidus (DI) rats with that from normal and water-deprived Long-Evans (LE) rats. A pool of atrial tissue extract (AE) was prepared from normal Sprague-Dawley rats for use as a standard against which unknown AE samples could be compared. Five doses, ranging from 27 to 432 micrograms of AE protein, were assayed in the Sprague-Dawley bioassay rats. Phosphate-buffered saline (PBS) vehicle and ventricular tissue extracts were also assayed. Statistical analysis of several log dose-response relationships revealed that the bioassay response most appropriate in determining relative natriuretic activity of AE was the log of the experimental\/control ratio for sodium excretion. The bioassay was used to demonstrate that PBS atrial extracts from both water-deprived LE rats and DI rats contain more natriuretic activity than do PBS atrial extracts from LE rats. <\/jats:p>","DOI":"10.1152\/ajprenal.1985.248.2.f314","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T19:43:27Z","timestamp":1513971807000},"page":"F314-F318","source":"Crossref","is-referenced-by-count":0,"title":["An improved bioassay method for determining natriuretic activity of atrial extracts"],"prefix":"10.1152","volume":"248","author":[{"given":"M. D.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.1985.248.2.F314","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T15:33:30Z","timestamp":1567956810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.1985.248.2.F314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,2,1]]}},"alternative-id":["10.1152\/ajprenal.1985.248.2.F314"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.1985.248.2.f314","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,2,1]]}}}