{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:21:24Z","timestamp":1648588884770},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[1992,10,1]]},"abstract":"<jats:p> This study examines the ontogeny of cellular pH regulation in renal proximal tubule cells (RPTC). RPTC from 8- to 40-day-old Sprague-Dawley rats (RPTC-8 to RPTC-40) were studied after 48 h of primary culture. Intracellular pH (pHi) was measured by quantitative fluorescence microscopy using 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. Recordings were made under basal conditions and after imposing a cytoplasmic alkalosis and acidosis using 15 mM NH4+ salt. The net recovery rate (dpHi\/dt) from intracellular acidosis increases significantly between 10 and 12 days of age from 0.39 +\/- 0.04 to 0.54 +\/- 0.06 pH units\/min (P &lt; 0.05, n = 10 vs. 6). This increase can be completely accounted for by an increase in the rate of amiloride (100 microM)-inhibitable Na(+)-H+ exchange (0.29 +\/- 0.04 vs. 0.42 +\/- 0.05 pH units\/min, P &lt; 0.05, n = 6 vs. 6). The rate of Na(+)-H+ exchange increases similarly in RPTC-10 and RPTC-40 when the transmembrane Na+ gradient is increased by Na+ depleting the cells (48 and 49%, respectively). The amiloride-insensitive recovery is Na+ independent and insensitive to 4-acetamido-4'-isothiocyanostilbene-2-2'-disulfonic acid (SITS, 500 microM) (range 0.08-0.14 pH units\/min). The net recovery rate from intracellular alkalosis is significantly lower in RPTC-10 than in RPTC-40 (0.16 +\/- 0.02 vs. 0.28 +\/- 0.02 pH units\/min, P &lt; 0.01, n = 4 vs. 5). SITS (500 microM) inhibits the recovery by 27 +\/- 8 and 26 +\/- 9%, respectively, whereas amiloride has no effect.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajprenal.1992.263.4.f716","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T04:42:36Z","timestamp":1514004156000},"page":"F716-F721","source":"Crossref","is-referenced-by-count":1,"title":["Intracellular pH regulation in cultured renal proximal tubule cells in different stages of maturation"],"prefix":"10.1152","volume":"263","author":[{"given":"H.","family":"Ekblad","sequence":"first","affiliation":[{"name":"Department of Pediatrics, St. Goran's Children's Hospital, KarolinskaInstitute, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Aperia","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, St. Goran's Children's Hospital, KarolinskaInstitute, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. H.","family":"Larsson","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, St. Goran's Children's Hospital, KarolinskaInstitute, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.1992.263.4.F716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:23:15Z","timestamp":1567959795000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.1992.263.4.F716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,10,1]]}},"alternative-id":["10.1152\/ajprenal.1992.263.4.F716"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.1992.263.4.f716","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10,1]]}}}