{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T22:46:15Z","timestamp":1649198775539},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[1997,6,1]]},"abstract":"<jats:p> Potassium depletion is involved in the pathophysiology of metabolic alkalosis. In the present study, the expression of renal acid-base transporters that are involved in HCO3- reabsorption was studied in potassium depletion. Rats fed potassium-deficient (KD) diet developed significant hypokalemia at 14 days (serum K+ 1.9 +\/- 0.2 in KD vs. 4.2 +\/- 0.2 meq\/l in control, P &lt; 0.01) but not at 6 days (3.8 +\/- 0.3 in KD vs. 4.1 +\/- 0.3 meq\/l in control, P &gt; 0.05). Kidney mRNA for colonic H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase, cHKA) increased by approximately 3- and 11-fold at 6 and 14 days of KD diet, respectively, indicating that increased expression preceded the onset of hypokalemia. The expression of Na+\/H+ exchanger 3 (NHE-3) mRNA and its cognate protein remained unchanged at 6 and 14 days of KD diet. The mRNA levels for NHE-1, NHE-2, and NHE-4 also remained unchanged at 6 and 14 days of KD diet. Hypophysectomized (HPX) rats fed KD diet for 14 days developed similar hypokalemia. However, the expression of cHKA mRNA in the kidney was decreased by approximately 80% in potassium-depleted (HPX+KD) rats (P &lt; 0.01 vs. KD only). Hypophysectomy did not affect the mRNA levels for either gastric H(+)-K(+)-ATPase (gHKA) or NHE isoforms in KD animals. Thus potassium depletion increases expression of cHKA in the kidney but not that of gHKA or NHE isoforms. The signal for this increase appears to precede hypokalemia. Furthermore, the data suggest that pituitary hormone(s) plays an important and novel role in the regulation of cHKA. <\/jats:p>","DOI":"10.1152\/ajprenal.1997.272.6.f736","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T07:50:33Z","timestamp":1514015433000},"page":"F736-F743","source":"Crossref","is-referenced-by-count":3,"title":["Potassium depletion and acid-base transporters in rat kidney: differential effect of hypophysectomy"],"prefix":"10.1152","volume":"272","author":[{"given":"Z.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Cincinnati School of Medicine, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Baird","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cincinnati School of Medicine, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Shumaker","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cincinnati School of Medicine, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cincinnati School of Medicine, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.1997.272.6.F736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:12:01Z","timestamp":1567973521000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.1997.272.6.F736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,6,1]]}},"alternative-id":["10.1152\/ajprenal.1997.272.6.F736"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.1997.272.6.f736","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,1]]}}}