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Gene expression was assessed by semiquantitative reverse transcription-polymerase chain reaction and Western blotting. COX-2 was highly expressed at the early stages of bladder development and declined progressively throughout gestation. In adult bladder, both COX-1 and COX-2 were detectable at low levels under basal conditions. An \u223c30-fold increase in COX-2 mRNA was seen after 24 h of obstruction. In contrast, COX-1 did not change with obstruction. COX-2 mRNA levels peaked at 6 h of obstruction. In regional bladder-distention models, COX-2 induction was confined to the area of distention. Bladder outlet obstruction stimulates COX-2 expression dramatically, reactivating a gene that is highly expressed during fetal development.<\/jats:p>","DOI":"10.1152\/ajprenal.1997.273.4.f538","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:56:01Z","timestamp":1514040961000},"page":"F538-F544","source":"Crossref","is-referenced-by-count":28,"title":["Cyclooxygenase-2 is expressed in bladder during fetal development and stimulated by outlet obstruction"],"prefix":"10.1152","volume":"273","author":[{"given":"John M.","family":"Park","sequence":"first","affiliation":[{"name":"Section of Urology, Department of Surgery;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianxin","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lois J.","family":"Arend","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann M.","family":"Smart","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurgen B.","family":"Schnermann","sequence":"additional","affiliation":[{"name":"Department of Physiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine P.","family":"Briggs","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine; and"},{"name":"Department of Physiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.2.C482"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1976.tb06711.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)55955-8"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90109-4"},{"key":"B5","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0022-3565(25)21541-5","volume":"230","author":"Downie J. 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