{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T07:10:11Z","timestamp":1765609811495},"reference-count":34,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:p>We have identified in rabbit renal cells two alternatively spliced transcripts of the \u03b1-subunit rbslo1 and rbslo2. Rbslo1 has a novel \u201cin-frame\u201d 174-bp insertion immediately after the predicted S8 transmembrane segment, whereas rbslo2 has a 104-bp deletion between S9 and S10, creating a frameshift and a premature termination codon. Amino acid identity between mouse maxi K<jats:sup>+<\/jats:sup>channel \u03b1-subunit ( mslo) and rbslo1 was 99%. Two transcript sizes of 4.2 and 7.5 kb were detected in brain, kidney, stomach, testis, and lung. Rbslo is expressed in glomeruli, thin limbs of Henle\u2019s loop, medullary and cortical thick ascending limbs of Henle\u2019s loop, and cortical, outer, and inner medullary collecting ducts; however, it was rarely detected in proximal convoluted tubules. Rbslo1 is most abundant in inner medulla. Expressed in Xenopus oocytes, rbslo1 generates depolarization-activated, outwardly rectifying K<jats:sup>+<\/jats:sup>currents. Rbslo1 expressed in Chinese hamster ovary cells could be activated by depolarization and Ca<jats:sup>2+<\/jats:sup>. These data suggest that rbslo transcripts are expressed in multiple nephron segments and that the magnitude of mRNA expression varies among different nephron segments.<\/jats:p>","DOI":"10.1152\/ajprenal.1997.273.4.f615","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:56:01Z","timestamp":1514040961000},"page":"F615-F624","source":"Crossref","is-referenced-by-count":24,"title":["Cloning and characterization of maxi K<sup>+<\/sup>channel \u03b1-subunit in rabbit kidney"],"prefix":"10.1152","volume":"273","author":[{"given":"Takashi","family":"Morita","sequence":"first","affiliation":[{"name":"Department of Physiology, Johns Hopkins University School of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazushige","family":"Hanaoka","sequence":"additional","affiliation":[{"name":"Department of Physiology, Johns Hopkins University School of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo M.","family":"Morales","sequence":"additional","affiliation":[{"name":"Department of Physiology, Johns Hopkins University School of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chahrzad","family":"Montrose-Rafizadeh","sequence":"additional","affiliation":[{"name":"National Institute on Aging, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William B.","family":"Guggino","sequence":"additional","affiliation":[{"name":"Department of Physiology, Johns Hopkins University School of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90160-F"},{"issue":"12","key":"B2","first-page":"L1057","volume":"268","author":"Alioua A.","year":"1995","journal-title":"Am. 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