{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:49:47Z","timestamp":1778276987604,"version":"3.51.4"},"reference-count":54,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2000,2,1]]},"abstract":"<jats:p>Previous studies have suggested that mineralocorticoids are needed for a normal action of vasopressin on collecting duct osmotic water permeability. However, the mechanisms behind this are unknown. To investigate if aldosterone-receptor blockade influences vasopressin type 2 receptor (V<jats:sub>2<\/jats:sub>)-mediated renal water reabsorption and the renal expression of the vasopressin-regulated water channel aquaporin-2 (AQP2), rats were treated with the aldosterone-receptor antagonist canrenoate (20 mg\/day iv) for 4 wk. Daily urine flow was increased significantly by 44%, and urine osmolality was decreased by 27% in canrenoate-treated rats. Acute V<jats:sub>2<\/jats:sub>-receptor blockade (OPC-31260, 800 \u03bcg \u22c5 kg<jats:sup>\u22121<\/jats:sup>\u22c5 h<jats:sup>\u22121<\/jats:sup>) was performed under conditions in which volume depletion was prevented. In control rats, OPC-31260 induced a significant increase in urine flow rate (V, +25%) and free water clearance ([Formula: see text], \u221229%). In canrenoate-treated rats, the effect of OPC-31260 was significantly reduced, and semiquantiative immunoblotting demonstrated a significant reduction (45%) in AQP2 expression. Because rats with common bile duct ligation (CBL) have a reduced vasopressin-mediated water reabsorption compared with normal rats (V: \u221224%;[Formula: see text]: \u221228%, and 86% downregulation of AQP2), the effect of canrenoate combined with OPC-31260 was tested. Canrenoate treatment of CBL rats significantly increased daily urine flow, decreased urine osmolality, and impaired the aquaretic response to OPC-31260 (V: \u221223%;[Formula: see text]: \u221231%) with maintained suppression of the renal AQP2 expression. Thus canrenoate treatment of normal and CBL rats showed 1) increased urine production, 2) reduced aquaretic effect of acute V<jats:sub>2<\/jats:sub>-receptor blockade, and 3) a marked reduction in AQP2 expression. This strongly supports the view that aldosterone plays a significant role for vasopressin-mediated water reabsorption.<\/jats:p>","DOI":"10.1152\/ajprenal.2000.278.2.f246","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T11:35:23Z","timestamp":1514028923000},"page":"F246-F256","source":"Crossref","is-referenced-by-count":31,"title":["Decreased vasopressin-mediated renal water reabsorption in rats with chronic aldosterone-receptor blockade"],"prefix":"10.1152","volume":"278","author":[{"given":"Thomas E. 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