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Steroid hormones 1) increased Na<jats:sup>+<\/jats:sup>transport by three- to fourfold, 2) increased the maximum capacity of the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>pump by 30\u201350%, 3) increased the steady-state levels of the \u03b1<jats:sub>1<\/jats:sub>-subunit of the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase by \u223c30%, and 4) increased the steady-state levels of the \u03b1-subunit of the rat epithelial Na<jats:sup>+<\/jats:sup>channel (\u03b1-rENaC) by nearly fourfold. TGF-\u03b2 blocked the effects of steroids on the increase in Na<jats:sup>+<\/jats:sup>transport and the stimulation of the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase and pump capacity. However, there was no effect of TGF-\u03b2 on the steroid-induced increase in mRNA levels of \u03b1-rENaC. The effects of TGF-\u03b2 were not secondary to the decrease in Na<jats:sup>+<\/jats:sup>transport per se, inasmuch as benzamil inhibited the increase in Na<jats:sup>+<\/jats:sup>transport but did not block the increase in pump capacity or Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-ATPase mRNA. The results indicate that TGF-\u03b2 does not inactivate the steroid receptor or its translocation to the nucleus. Rather, they indicate complex pathways involving interruption of the enhancement of pump activity and activation\/inactivation of pathways distal to the steroid-induced increase in the transcription of \u03b1-rENaC.<\/jats:p>","DOI":"10.1152\/ajprenal.2000.278.3.f425","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T12:57:46Z","timestamp":1514033866000},"page":"F425-F433","source":"Crossref","is-referenced-by-count":20,"title":["Mechanisms of inactivation of the action of aldosterone on collecting duct by TGF-\u03b2"],"prefix":"10.1152","volume":"278","author":[{"given":"Russell F.","family":"Husted","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Iowa, and Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita D.","family":"Sigmund","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Iowa, and Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John B.","family":"Stokes","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Iowa, and Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"7799","DOI":"10.1016\/S0021-9258(19)38999-9","volume":"265","author":"Barlet-Bas C.","year":"1990","journal-title":"J. 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