{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:41:05Z","timestamp":1791862865127,"version":"4.3.4"},"reference-count":71,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:p>We studied the role of the C5b-9 membrane attack complex in two models of inflammatory glomerulonephritis (GN) initiated by acute glomerular endothelial injury in Piebold-viral-Glaxo (PVG) complement-sufficient rats (C+), C6-deficient rats (C6\u2212), and rats systematically depleted of complement with cobra venom factor (CVF). GN was induced by performing a left nephrectomy and selectively perfusing the right kidney with either 1) the lectin concanavalin A (Con A) followed by complement-fixing anti-Con A (Con A GN) or 2) purified complement-fixing goat anti-rat glomerular endothelial cell (GEN) antibody [immune-mediated thrombotic microangiopathy (ITM)]. Comparable levels of GEN apoptosis were detected in C+ animals in both models. CVF administration reduced GEN apoptosis by 10- to 12-fold. GEN apoptosis was C5b-9 dependent because PVG C6\u2212 rats were protected from GEN loss. Furthermore, functional inhibition of the cell surface complement regulatory protein CD59 by renal perfusion with anti-CD59 antibody in ITM resulted in a 3.5-fold increase in GEN apoptosis. Last, in Con A GN, abrogation of GEN apoptosis preserved endothelial integrity and renal function. This study demonstrates the specific role of C5b-9 in the induction of GEN apoptosis in experimental inflammatory GN, a finding with implications for diseases associated with the presence of antiendothelial cell antibodies.<\/jats:p>","DOI":"10.1152\/ajprenal.2000.278.5.f747","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T12:47:32Z","timestamp":1514033252000},"page":"F747-F757","source":"Crossref","is-referenced-by-count":57,"title":["C5b-9 membrane attack complex mediates endothelial cell apoptosis in experimental glomerulonephritis"],"prefix":"10.1152","volume":"278","author":[{"given":"Jeremy","family":"Hughes","sequence":"first","affiliation":[{"name":"Division of Nephrology, Departments of Medicine and Pathology, University of Washington, Seattle, Washington, 98195; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaomi","family":"Nangaku","sequence":"additional","affiliation":[{"name":"University of Tokyo, Tokyo 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