{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:22:54Z","timestamp":1778275374390,"version":"3.51.4"},"reference-count":40,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:p>To test the proposal that the enzyme 11\u03b2-hydroxysteroid dehydrogenase (11\u03b2-HSD) confers aldosterone specificity on mineralocorticoid receptors in the distal nephron by inactivating glucocorticoids, we performed a free-flow micropuncture study of distal tubular function in adrenalectomized rats infused with high-dose corticosterone. One-half of the rats were additionally given intravenous carbenoxolone (CBX; 6 mg\/h) to inhibit renal 11\u03b2-HSD activity. Although this maneuver lowered fractional Na<jats:sup>+<\/jats:sup>excretion (1.1 \u00b1 0.2 vs. 1.9 \u00b1 0.2%, P &lt; 0.01), Na<jats:sup>+<\/jats:sup>reabsorption within the accessible distal tubule was found to be similar in the two groups of animals. In contrast, distal tubular K<jats:sup>+<\/jats:sup>secretion was enhanced in CBX-treated rats: fractional K<jats:sup>+<\/jats:sup>deliveries to the early and late distal collection sites in the corticosterone-alone group were 13 \u00b1 1 and 20 \u00b1 3%, respectively (not significant), whereas corresponding data in the CBX-treated group were 9 \u00b1 1 and 24 \u00b1 2% ( P &lt; 0.01). This stimulation of distal K<jats:sup>+<\/jats:sup>secretion provides the first direct in vivo evidence that 11\u03b2-HSD normally prevents corticosterone from exerting a mineralocorticoid-like effect in the distal tubule. The reduction in fractional Na<jats:sup>+<\/jats:sup>excretion during inhibition of 11\u03b2-HSD, in the absence of a change in end-distal Na<jats:sup>+<\/jats:sup>delivery, suggests enhanced Na<jats:sup>+<\/jats:sup>reabsorption in the collecting ducts.<\/jats:p>","DOI":"10.1152\/ajprenal.2001.280.1.f172","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T13:34:15Z","timestamp":1514036055000},"page":"F172-F179","source":"Crossref","is-referenced-by-count":16,"title":["Distal tubular electrolyte transport during inhibition of renal 11\u03b2-hydroxysteroid dehydrogenase"],"prefix":"10.1152","volume":"280","author":[{"given":"Katharine J.","family":"Biller","sequence":"first","affiliation":[{"name":"Division of Biomedical Sciences, Imperial College School of Medicine, Charing Cross Hospital, London W6 8RF; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert 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