{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T03:49:45Z","timestamp":1793936985933,"version":"4.8.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Renal Physiology"],"published-print":{"date-parts":[[2001,6,1]]},"abstract":"<jats:p> Adrenomedullin (AM) is a potent vasodilator and natriuretic peptide that plays an important role in cardiovascular function. In this study, we employed a somatic gene delivery approach to explore its potential protective role in renovascular hypertension. A single tail vein injection of adenovirus harboring the human AM gene significantly blunted a blood pressure increase that lasted for more than 3 wk in two-kidney one-clip (2K1C) hypertensive rats. The expression of human AM mRNA was detected in the kidney, adrenal gland, heart, lung, and liver, and immunoreactive human AM was detected in the plasma and urine of 2K1C rats after human AM gene delivery. A maximal blood pressure difference of 28 mmHg was observed 10 days after AM gene delivery, compared with that in rats injected with the control virus carrying the LacZ gene. Human AM gene delivery significantly attenuated increases in the ratio of left ventricular weight to heart weight, cardiomyocyte diameter, and fibrosis in the heart, as well as glomerular sclerosis, tubular injuries, and protein casts in the kidney. The beneficial effects of AM gene delivery were accompanied by increased urinary cAMP levels, indicating activation of AM receptors. These findings provide new insights into the role of AM in renovascular hypertension and may have significance in therapeutic applications in cardiovascular diseases. <\/jats:p>","DOI":"10.1152\/ajprenal.2001.280.6.f964","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:14:11Z","timestamp":1514038451000},"page":"F964-F971","source":"Crossref","is-referenced-by-count":48,"title":["Adrenomedullin gene delivery attenuates renal damage and cardiac hypertrophy in Goldblatt hypertensive rats"],"prefix":"10.1152","volume":"280","author":[{"given":"Cindy","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dobrzynski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(99)00025-7"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.20.269"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90524-X"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005604200"},{"key":"B5","first-page":"37","volume":"13","author":"Gettys TW","year":"1990","journal-title":"Second Messengers Phosphoproteins"},{"key":"B6","first-page":"207","volume":"1","author":"Harper JF","year":"1975","journal-title":"J Cyclic Nucleotide Res"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2509"},{"key":"B8","first-page":"138","volume":"21","author":"Hinson JP","year":"2000","journal-title":"Endocr Rev"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(99)00559-2"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)80106-1"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90214-3"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1995.268.4.F657"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01136-9"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1451"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1881"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.275.3.F410"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1038\/30666"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.1998.00855.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00400-2"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.24.5.600"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.2241"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00928-7"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.2132"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.363"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.19.2309"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.1.505"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.24.6.770"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jhh.1000747"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.5.1104"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3109\/07853899609092931"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00475-0"}],"container-title":["American Journal of Physiology-Renal Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajprenal.2001.280.6.F964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:56:57Z","timestamp":1567976217000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajprenal.2001.280.6.F964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6,1]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2001,6,1]]}},"alternative-id":["10.1152\/ajprenal.2001.280.6.F964"],"URL":"https:\/\/doi.org\/10.1152\/ajprenal.2001.280.6.f964","relation":{},"ISSN":["1931-857X","1522-1466"],"issn-type":[{"value":"1931-857X","type":"print"},{"value":"1522-1466","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,6,1]]}}}